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Modulations of rabbit erythrocyte ATPase activities induced by in vitro and in vivo exposure to ethanol

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Abstract

Alcohol intake is associated with numerous degenerative disorders, and the detrimental effects of alcohol may be due to its influence on plasma membrane and cellular transport systems. The aim of the present study was to compare in vitro and in vivo effects of ethanol on rabbit erythrocyte ATPase activities and correlate them with ethanol-induced oxidative stress. Age-matched male rabbits were given 5% ethanol in 2% sucrose solution, for 6 weeks ad libitum; control animals were given tap water. Daily intake of ethanol was 5 g/kg body weight; this experimental regimen resulted in an average serum ethanol concentration of 16.77 ± 2.00 mM/l. After this period, blood was collected, serum ethanol concentration was determined and erythrocyte membranes were prepared according to the method of Post et al. Activities of Na+/K+- and Mg2+-ATPases were determined. Thiobarbituric acid-reactive substance (TBARS) assay was used to detect levels of lipid peroxidation, a major indicator of oxidative stress. In vitro ethanol inhibits both Na+/K+-ATPase and Mg2+-ATPase, but Na+/K+-ATPase is more sensitive to the ethanol-induced inhibition. Increasing concentration of ethanol increased TBARS production, but significant difference was attained only at 5 and 12.5 mM of ethanol. Chronic ethanol consumption induced significant increase in Na+/K+- and Mg2+-ATPase activity, and TBARS production. Our results suggest that increased ATPase activity induced by chronic ethanol consumption is due to oxidative, induced modification of membrane phospholipids and proteins, which are responsible for inhibition of ATPase activity. Increased production of TBARS induced by in vitro exposure to ethanol is not the only factor that influences ATPases activity. Further research is needed to elucidate this relationship.

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Acknowledgements

This work was supported by the Ministry for Science, Technology and Environmental Protection of Serbia, Grant #145029B.

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Authors and Affiliations

  1. Institute of Physiology, School of Medicine, University of Belgrade, Višegradska 26/II, 11000, Belgrade, Serbia

    Aleksandra Rašić-Marković, Olivera Stanojlović, Dragan Hrnčić, Dragan Djurić & Helena Lončar-Stevanović

  2. Institute of Clinical Chemistry, School of Medicine, University of Belgrade, Belgrade, Serbia

    Danijela Krstić & Zorana Vujović

  3. Department of Physiology, School of Medicine, University of Kragujevac, Kragujevac, Serbia

    Vladimir Jakovljevic

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  1. Aleksandra Rašić-Marković
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  2. Danijela Krstić
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  5. Olivera Stanojlović
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Correspondence to Olivera Stanojlović.

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Rašić-Marković, A., Krstić, D., Vujović, Z. et al. Modulations of rabbit erythrocyte ATPase activities induced by in vitro and in vivo exposure to ethanol. Mol Cell Biochem 308, 111–116 (2008). https://doi.org/10.1007/s11010-007-9618-z

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  • DOI: https://doi.org/10.1007/s11010-007-9618-z

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