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Radiosensitization by diospyrin diethylether in MCF-7 breast carcinoma cell line

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Abstract

The development of radio-resistant tumor cells might be overcome by the use of tumor selective cytotoxic agents in combination with radiation treatment of cancer. Thus, we are exploring the radiomodifying potential of D7, a tumor-inhibitory compound derived from a plant product, diospyrin, in breast carcinoma cells, MCF-7. The present study indicated that D7 could enhance the radiation-induced cytotoxicity and apoptosis through down-regulation of the anti-apoptotic Bcl-2 and COX-2 gene expression, and up-regulation of pro-apoptotic genes, like p53 and p21. The higher expression of PUMA, a pro-apoptotic protein was also observed in the combination treatment. Effect of D7 on up-regulation of p21 expression in irradiated MCF-7 cells was concomitant with the cell cycle arrest in the G1 phase. Thus, it was concluded that D7 could sensitize the effect of radiation in breast carcinoma by regulating the gene expression involved in cell cycle and apoptosis.

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Acknowledgment

The work was supported by the Board of Radiation and Nuclear Sciences, Department of Atomic Energy, Mumbai (Research Grant no. 2002/37/19/BRNS), and UGC, New Delhi (BH). The authors acknowledge Shri S. Jayakumar and Dr. R.C. Chaubey of Radiation Biology and Health Sciences Division, BARC, Mumbai, for providing the facility and helpful discussion for Comet assay. The authentic stem bark of Diospyros montana Roxb. was collected by Dr. R.B. Mishra, Nagarjuna Education Society, Bolangir, Orissa.

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Authors and Affiliations

  1. Department of Pharmaceutical Technology, Jadavpur University, Raja S.C. Mullick Road, Jadavpur, Calcutta, West Bengal, 700032, India

    Binod Kumar & Banasri Hazra

  2. Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Mumbai, 400 085, India

    Jayashree Joshi, Amit Kumar, Badri N. Pandey & Kaushala P. Mishra

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  1. Binod Kumar
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  2. Jayashree Joshi
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  3. Amit Kumar
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  4. Badri N. Pandey
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  5. Banasri Hazra
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Correspondence to Banasri Hazra.

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Kumar, B., Joshi, J., Kumar, A. et al. Radiosensitization by diospyrin diethylether in MCF-7 breast carcinoma cell line. Mol Cell Biochem 304, 287–296 (2007). https://doi.org/10.1007/s11010-007-9511-9

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  • DOI: https://doi.org/10.1007/s11010-007-9511-9

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