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Anticancer Activity of Brevinin-2R Peptide and its Two Analogues Against Myelogenous Leukemia Cell Line as Natural Treatments: An In Vitro Study

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Abstract

Human chronic myelogenous leukemia (CML) is a stem cell driven hematological malignancy which shows resistance to existing therapeutics. This property of CML accentuates the necessity to develop alternative anti-CML therapeutic agents. Herein, we have evaluated the anticancer activity of a novel anticancer peptide, Brevinin-2R and its two analogues, Brevinin-2R-C and Brevinin-2R-D regarding their inhibitory activity against K562 cells. Various cell-based analyses have been conducted to analyze the effects of these peptides and their mechanism of action. Hematotoxicity assay was performed to determine their hemolytic activities. MTT and neutral red uptake assays were conducted to examine anti-proliferative effects, propidium iodide (PI) staining to monitor the DNA content in different phases of cell cycle and Annexin V/PI staining to detect the apoptosis induction for the peptides. Our findings indicated that these peptides are capable of diminishing the cell growth and inducing apoptosis and cell cycle arrest. Brevinin-2R and its two analogues inhibited cell proliferation through strong cell cycle arrest in G2/M phase leading to apoptosis induction. The cytotoxicity of Brevinin-2R was higher than that of its two derivatives. These observations have provided new insights into the therapeutic activity of Brevinin-2R and its two analogues and suggest that these peptides have the potential to act as anticancer agents in treatment of K562. Further in vivo investigations on the therapeutic potential of Brevinin-2R and its two analogues are required to get a better grasp of their mechanism of action.

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Acknowledgements

The authors wish to thank Islamic Azad University of Ardabil for supporting the conduct of this research. This study was supported by Grant No. 9775 from Shahroud University of Medical Sciences.

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Authors and Affiliations

  1. Department of Biology, Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, Iran

    Robab Hassanvand Jamadi

  2. Department of Biology Sciences, Shahid Rajaee Teacher Training University, Tehran, Iran

    Saeed Khalili

  3. Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran

    Tooba Mirzapour

  4. Department of Biology, Ardabil Branch, Islamic Azad University, Ardabil, Iran

    Robab Hassanvand Jamadi & Hashem Yaghoubi

  5. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Zahra Sadat Hashemi

  6. Department of Clinical Biochemistry, Faculty of Medical Sciences, Dezful University of Medical Sciences, Dezful, Iran

    Maysam Mard-Soltani

  7. Clinical Biochemistry, Cancer Prevention Research Center, Shahroud University of Medical Sciences, Shahroud, Iran

    Moslem Jafarisani

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  1. Robab Hassanvand Jamadi
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  2. Saeed Khalili
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  4. Hashem Yaghoubi
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Correspondence to Hashem Yaghoubi.

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Hassanvand Jamadi, R., Khalili, S., Mirzapour, T. et al. Anticancer Activity of Brevinin-2R Peptide and its Two Analogues Against Myelogenous Leukemia Cell Line as Natural Treatments: An In Vitro Study. Int J Pept Res Ther 26, 1013–1020 (2020). https://doi.org/10.1007/s10989-019-09903-6

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