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Antibacterial Natural Peptide Fractions from Indian Ganoderma lucidum

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Abstract

Natural peptides are emerging as a leading alternative to conventional drugs and antibiotics, owing to their remarkable potency, better stability and less toxicity. Such peptides encompass numerous healing properties such as antimicrobial, anti-inflammatory, immunomodulatory, etc. Though plant- derived peptides have been widely studied for their therapeutic benefits, however, fungal peptides are still lesser explored. Ganoderma lucidum, a highly medicinal oriental mushroom comprises a vast array of phytoconstituents, namely flavonoids, phenolics, terpenoids, polysaccharides, proteins, glycolipids, etc and hence, is being used since several decades in traditional Chinese medicine (TCM) for its various ameliorative effects e.g. anti-inflammatory, antimicrobial, anti-proliferative and antioxidant properties. This study presents the isolation and characterization of antibacterial peptide fractions from fruiting body (GLF) and mycelium (GLM) of Indian G. lucidum. Representative amide bonds were identified in the fractions using established standard techniques. Peptide mass fingerprinting and HPLC confirmed the presence of cationic and hydrophobic amino acids in the peptide fractions which are known to be major structural features of antimicrobial peptides. Secondary structure prediction showed abundance of α-helices and random coils in GLF and GLM fractions respectively. The fractions exhibited appreciable antioxidant potential. Besides, these also possessed substantial antibacterial activity against Escherichia coli and Salmonella typhi wherein it was observed that generation of reactive oxygen species and induction of intracellular protein leakage within the bacterial cells were the possible mechanisms of inhibitory action.

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Acknowledgements

The authors would like to express their gratitude to the Director, DIPAS for his constant support and encouragement. The authors would also extend their thanks to Advanced Research Facility, Jawaharlal Nehru University, Delhi and Department of Chemistry, Delhi University for providing analytical support. Two of the authors Miss Jigni Mishra and Miss Rakhee would like to thank Defence Research and Development Organization, India and Council for Scientific and Industrial Research, India, respectively, for doctoral fellowships.

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Authors and Affiliations

  1. Defence Institute of Physiology and Allied Sciences, Delhi, India

    Jigni Mishra, Rakhee Rajput, Kaushlesh Singh, Anju Bansal & Kshipra Misra

  2. Department of Biotechnology, University Institute of Engineering and Technology, Panjab University, Chandigarh, India

    Sanjeev Puri

  3. Dr SS Bhatnagar University Institute of Chemical Engineering and Technology, Panjab University, Chandigarh, India

    Meenakshi Goyal

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  1. Jigni Mishra
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  2. Rakhee Rajput
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Correspondence to Kshipra Misra.

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Mishra, J., Rajput, R., Singh, K. et al. Antibacterial Natural Peptide Fractions from Indian Ganoderma lucidum . Int J Pept Res Ther 24, 543–554 (2018). https://doi.org/10.1007/s10989-017-9643-z

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