Abstract
Magic-angle spinning (MAS) is mandatory in solid-state NMR experiments to achieve resolved spectra. In rare cases, instabilities in the rotation or damage of either the rotor or the rotor cap can lead to a so called “rotor crash” involving a disintegration of the sample container and possibly the release of an aerosol or of dust. We present a modified design of a 3.2 mm probe with a confining chamber which in case of a rotor crash prevents the release of aerosols and possibly hazardous materials. 1D and 2D NMR experiments show that such a hazardous material-confining MAS probe (“CONFINE-MAS” probe) has a similar sensitivity compared to a standard probe and performs equally well in terms of spinning stability. We illustrate the CONFINE-MAS probe properties and performance by application to a fungal amyloid.
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Acknowledgements
This work was supported by the Swiss National Science Foundation (Grant 200020_159707), by the French ANR (ANR-11-LABX-0048 through ANR-11-IDEX-0007), and the ETH Career SEED-69 16-1. This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (Grant agreement no. 741863, FASTER).
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Wiegand, T., Hunkeler, A., Däpp, A. et al. CONFINE-MAS: a magic-angle spinning NMR probe that confines the sample in case of a rotor explosion. J Biomol NMR 72, 171–177 (2018). https://doi.org/10.1007/s10858-018-0218-x
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DOI: https://doi.org/10.1007/s10858-018-0218-x