Abstract
Water suppression by selective preirradiation is increasingly difficult to achieve on probeheads with high quality factor because of the opposing forces of radiation dam**. Here we show that a simple modification to the WET scheme provides reliable water suppression in aqueous solutions of proteins and peptides with minimal saturation of the Hα protons. The scheme is shown to work also with dilute peptide solutions. It is recommended to maintain the water suppression during the evolution time of COSY experiments by weak selective irradiation that causes only minimal Bloch-Siegert shifts. The new water-suppression scheme suppresses the water magnetization by spatial scrambling. Traditional water suppression by preirradiation is similarly based more on water scrambling due to the radiofrequency inhomogeneity than on relaxation effects.
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Abbreviations
- DANTE:
-
delays alternating with nutations for tailored excitation
- rf:
-
radiofrequency
- SWET:
-
secure WET
- WANTED:
-
Water selective DANTE using gradient
- WET:
-
water suppression enhanced through T1 effects
References
Abragam, A. (1961) The Principles of Nuclear Magnetism, Oxford University Press
W.P. Aue E. Bartholdi R.R. Ernst (1976) J. Chem. Phys. 64 2229–2246 Occurrence Handle10.1063/1.432450
M.P. Augustine (2002) Prog. NMR Spectrosc. 40 111–150 Occurrence Handle10.1016/S0079-6565(01)00037-1
A. Böckmann E. Guittet (1996) J. Biomol. NMR 8 87–92
P. Broekert J. Jeneer (1995) J. Magn. Reson. A 113 60–64 Occurrence Handle10.1006/jmra.1995.1056
I.D. Campbell C.M. Dobson G. Jeminet R.J. Williams (1974) FEBS Lett. 49 115–119 Occurrence Handle10.1016/0014-5793(74)80645-9 Occurrence Handle4442586
J.H. Chen A. Jerschow G. Bodenhausen (1999) Chem. Phys. Lett. 308 397–402 Occurrence Handle10.1016/S0009-2614(99)00596-5
B. Cutting J.H. Chen D. Moskau G. Bodenhausen (2000) J. Biomol. NMR 17 323–320 Occurrence Handle10.1023/A:1008303322609 Occurrence Handle11014596
A.L. Davis E.D. Laue J. Keeler D. Moskau J. Lohman (1991) J. Magn. Reson. 94 637–644
V.P. Denisov B. Halle (2002) J. Am. Chem. Soc. 124 10264–10265
M. Guéron P. Plateau M. Decorps (1991) Prog. NMR Spectrosc. 23 135–209 Occurrence Handle10.1016/0079-6565(91)80007-O
D.I. Hoult (1976) J. Magn. Reson. 21 337–347
R.E. Hurd (1990) J. Magn. Reson. 87 422–428
B.K. John D. Plant P. Webb R.H. Hurd (1992) J. Magn. Reson. 98 200–206
A. Louis-Joseph D. Abergel J.-Y. Lallemand (1995) J. Biomol. NMR 5 212–216 Occurrence Handle10.1007/BF00208813
W.E. Maas F.H. Laukien D.G. Cory (1995) J. Magn. Reson. A 113 274–277 Occurrence Handle10.1006/jmra.1995.1094
X.A. Mao J.X. Guo C.H. Ye (1994) Phys. Rev. B 49 15702–15711 Occurrence Handle10.1103/PhysRevB.49.15702
R.J. Ogg P.B. Kingsley J.S. Taylor (1994) J. Magn. Reson. Ser. B 104 1–10 Occurrence Handle10.1006/jmrb.1994.1048
Y. Ohtomo T. Bergman B.L. Johansson H. Jörnvall J. Wahren (1998) Diabetologia 41 287–291 Occurrence Handle10.1007/s001250050905 Occurrence Handle9541168
G. Otting (1994) J. Magn. Reson. B 103 288–291 Occurrence Handle10.1006/jmrb.1994.1043
G. Otting (1997) Prog. NMR Spectrosc. 31 259–285 Occurrence Handle10.1016/S0079-6565(97)00012-5
G. Otting E. Liepinsh (1995a) J. Biomol. NMR 5 420–426 Occurrence Handle10.1007/BF00182286
G. Otting E. Liepinsh (1995b) J. Magn. Reson. B 107 192–196 Occurrence Handle10.1006/jmrb.1995.1078
G. Otting K. Wüthrich (1988) J. Magn. Reson. 76 569–574
U. Piantini O.W. Sørensen R.R. Ernst (1982) J. Am. Chem. Soc. 104 6800–6801
M. Piotto V. Saudek V. Sklenář (1992) J. Biomol. NMR 2 661–665 Occurrence Handle1490109
W.S. Price (1999) Ann. Rep. NMR Spectrosc. 38 289–354
N.F. Ramsey (1955) Phys. Rev. 100 1191–1194 Occurrence Handle10.1103/PhysRev.100.1191
J.C. Rodriguez P.A. Jennings G. Melacini (2002) J. Am. Chem. Soc. 124 6240–6241
V. Sklenář M. Piotto R. Leppik V. Saudek (1993) J. Magn. Reson. A 102 241–245 Occurrence Handle10.1006/jmra.1993.1098
S.H. Smallcombe S.L. Patt P.A. Keifer (1995) J. Magn. Reson. A 117 295–303 Occurrence Handle10.1006/jmra.1995.0759
H.C. Torrey (1949) Phys. Rev. 76 1059–1068 Occurrence Handle10.1103/PhysRev.76.1059
P.C.M. Zijl Particlevan M.O. Johnson S. Mori R.E. Hurd (1995) J. Magn. Reson. A 113 265–270 Occurrence Handle10.1006/jmra.1995.1092
A. Vlassenbroek J. Jeener P. Broekert (1995) J. Chem. Phys. 103 5886–5897 Occurrence Handle10.1063/1.470468
G. Wider R.V. Hosur K. Wüthrich (1983) J. Magn. Reson. 52 130–135
K. Wüthrich (1986) NMR of Proteins and Nucleic Acids Wiley New York
S.M. Zhang D.G. Gorenstein (1996) J. Magn. Reson. A 118 291–294 Occurrence Handle10.1006/jmra.1996.0040
E.R.P. Zuiderweg (1987) J. Magn. Reson. 71 283–293
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Wu, P.S.C., Otting, G. SWET for Secure Water Suppression on Probes with High Quality Factor. J Biomol NMR 32, 243–250 (2005). https://doi.org/10.1007/s10858-005-8531-6
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DOI: https://doi.org/10.1007/s10858-005-8531-6