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Enhanced biosafety of silica coated gadolinium based nanoparticles

  • Clinical Applications of Biomaterials
  • Original Research
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Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

One of the most important and novel approaches of biomedical engineering is the development of new, effective and non-invasive medical diagnosis abilities, and treatments that have such requirements as advanced technologies for tumor imaging. Gadolinium (Gd) compounds can be used as MRI contrast agents, however the release of Gd3+ ions presents some adverse side effects such as renal failure, pancreatitis or local necrosis. The main aim of the work was the development and optimization of Gadolinium based nanoparticles coated with silica to be used as bioimaging agent. Gd based nanoparticles were prepared through a precipitation method and afterwards, these nanoparticles were covered with silica, using Stöber method with ammonia and functionalized with 3-Aminopropyltriethoxysilane (APTES). Results showed that nanoparticles were homogeneous regarding chemical composition, silica layer thickness, total size and morphology. Also, silica coating was successfully not degraded after 4 weeks at pH 5.5, 6.0 and 7.4, contrary to GdOHCO3 nanoparticles that degraded. Regarding the in vitro cell tests, very good cell proliferation and viability were observed. In conclusion, the results showed that Gd based nanoparticles coated with silica for imaging applications were successfully obtained under a well-controlled method. Furthermore, silica coating may enhance magnetic nanoparticles biosafety because it avoids GdOHCO3 degradation into harmful products (such as Gd3+ ions) at physiological conditions.

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Acknowledgements

The authors would like to acknowledge Dr. Yoshimitsu Kuwahara from Graduate School of Life Science and Systems Engineering at Kyushu Institute of Technology and Dr. Eiji Fujii from Industrial Technology Center of Okayama Prefecture for their support in TEM analysis. They also would like to acknowledge Prof. Masaki Mito from Faculty of Engineering at Kyushu Institute of Technology and Center for Instrumental Analysis, Kyushu Institute of Technology, for his support in SQUID analysis; Prof. Satoshi Hayakawa and Dr. Tomohiko Yoshioka from Graduate School of Natural and Science Technology at Okayama University for their support in SAA analysis and Dr. Anabela Dias and department of Física Médica from IPO—Instituto Português de Oncologia Dr. Francisco Gentil-centro Regional do Norte, for the help with MR images. This work was financed by FEDER—Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020—Operacional Programme for Competitiveness and Internationalisation (POCI), Portugal 2020, and by Portuguese funds through FCT—Fundação para a Ciência e a Tecnologia/Ministério da Ciência, Tecnologia e Inovação in the framework of the project “Institute for Research and Innovation in Health Sciences” (POCI-01-0145-FEDER-007274). Authors would also like to thank to SFRH/BPD/87713/2012 individual post-doctoral fellowship supported by FCT. The work was also co-financed by the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan through the program “Promotion and Standardization of the Tenure-Track System (Ko**senbatsu)”.

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Authors and Affiliations

  1. i3S, Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal

    Marta Laranjeira & Fernando Jorge Monteiro

  2. INEB, Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal

    Marta Laranjeira & Fernando Jorge Monteiro

  3. Faculdade de Engenharia, DEMM, Universidade do Porto, Porto, Portugal

    Marta Laranjeira & Fernando Jorge Monteiro

  4. Frontier Research Academy for Young Researchers, Kitakyushu-shi, Fukuoka, Japan

    Yuki Shirosaki & Saki Yoshimatsu Yasutomi

  5. Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, Kyushu, Fukuoka, Japan

    Toshiki Miyazaki

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  1. Marta Laranjeira
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  2. Yuki Shirosaki
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  3. Saki Yoshimatsu Yasutomi
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  4. Toshiki Miyazaki
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  5. Fernando Jorge Monteiro
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Correspondence to Marta Laranjeira.

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Laranjeira, M., Shirosaki, Y., Yoshimatsu Yasutomi, S. et al. Enhanced biosafety of silica coated gadolinium based nanoparticles. J Mater Sci: Mater Med 28, 46 (2017). https://doi.org/10.1007/s10856-017-5855-1

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  • DOI: https://doi.org/10.1007/s10856-017-5855-1

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