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Enhanced osteogenic differentiation of cord blood-derived unrestricted somatic stem cells on electrospun nanofibers

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Abstract

A new stem cell-scaffold construct based on poly-l-lactide (PLLA) nanofibers grafted with collagen (PLLA-COL) and cord blood-derived unrestricted somatic stem cells (USSC) were proposed to hold promising characteristics for bone tissue engineering. Fabricated nanofibers were characterized using SEM, ATR-FTIR, tensile and contact angle measurements. The capacity of PLLA, plasma-treated PLLA (PLLA-pl) and PLLA-COL scaffolds to support proliferation and osteogenic differentiation of USSC was evaluated using MTT assay and common osteogenic markers such as alkaline phosphatase (ALP) activity, calcium mineral deposition and bone-related genes. All three scaffolds showed nanofibrous and porous structure with suitable physical characteristics. Higher proliferation and viability of USSC was observed on PLLA-COL nanofibers compared to control surfaces. In osteogenic medium, ALP activity and calcium deposition exhibited the highest values on PLLA-COL scaffolds on days 7 and 14. These markers were also greater on PLLA and PLLA-pl compared to TCPS. Higher levels of collagen I, osteonectin and bone morphogenetic protein-2 were detected on PLLA-COL compared to PLLA and PLLA-pl. Runx2 and osteocalcin were also expressed continuously on all scaffolds during induction. These observations suggested the enhanced proliferation and osteogenic differentiation of USSC on PLLA-COL nanofiber scaffolds and introduced a new combination of stem cell-scaffold constructs with desired characteristics for application in bone tissue engineering.

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Authors and Affiliations

  1. Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran

    Ehsan Seyedjafari & Nasser Ghaemi

  2. Stem Cell Biology Department, Stem Cell Technology Research Center, Tehran, Iran

    Ehsan Seyedjafari

  3. Hematology Department, Faculty of Medical Science, Tarbiat Modares University, P.O. Box 14115-111, Tehran, Iran

    Masoud Soleimani

  4. School of Chemistry, College of Science, University of Tehran, Tehran, Iran

    Nasser Ghaemi

  5. Institute of Biophysics and Biochemistry, University of Tehran, Tehran, Iran

    Mohammad Nabi Sarbolouki

Authors
  1. Ehsan Seyedjafari
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  2. Masoud Soleimani
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  3. Nasser Ghaemi
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  4. Mohammad Nabi Sarbolouki
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Correspondence to Masoud Soleimani.

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Mohammad Nabi Sarbolouki deceased in 2009.

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Seyedjafari, E., Soleimani, M., Ghaemi, N. et al. Enhanced osteogenic differentiation of cord blood-derived unrestricted somatic stem cells on electrospun nanofibers. J Mater Sci: Mater Med 22, 165–174 (2011). https://doi.org/10.1007/s10856-010-4174-6

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  • DOI: https://doi.org/10.1007/s10856-010-4174-6

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