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Evaluation of the release rate of bioactive recombinant human epidermal growth factor from crosslinking collagen sponges

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Abstract

The purpose of this study was to prepare recombinant human epidermal growth factor (rhEGF) collagen sponges for topical applications and investigate the effects of different types of crosslinked collagen sponges as platforms for the controlled release of rhEGF. The microstructure and the drug release rates of collagen sponges were modified through treatment with different types (glutaraldehyde (GTA), genipin and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC)), different concentrations of crosslinking agents and various preparation conditions. A controlled release profile was observed for the crosslinked collagen sponges as compared to the non-crosslinked ones. The results indicated that the GTA crosslinked sponges have the most potent controlling effect. As the amount of GTA increased, a greater rigidity of the collagen sponge structure combined with a lower hydrophilicity was observed, leading to a decreased drug release rate and an increased water uptake. This study also demonstrated that a good correlation was obtained for in vitro release rates of rhEGF using the power model. The crosslinked rhEGF collagen sponges showed a successful delivery of rhEGF in bioactive form to stimulate cell proliferation.

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References

  1. G. CARPENTER and S. COHEN, Ann. Rev. Biochem. 48 (1979) 193

    Article  CAS  Google Scholar 

  2. I. K. COHEN, M. C. CROSSLAND, A. GARRETT and R. F. DIEGELMANN, Plast. Reconstr. Surg. 96 (1995) 251

    CAS  Google Scholar 

  3. J. M. BOWER, R. CAMBLE, H. GREGORY, E. L. GERRING and I. R. WILLSHIRE, Experientia 31 (1975) 825

    Article  CAS  Google Scholar 

  4. G. L. BROWN, G. SCHULTZ, J. R. BRIGHTWELL and G. R. TOBIN, Surg. Forum. 35 (1984) 565

    Google Scholar 

  5. G. L. BROWN, L. CURTSINGER, J. R. BRIGHTWELL, D. M. ACKERMAN, G. R. TOBIN, H. C. POLK, C. GEORGE-NASCIMENTO, P. VALENZUELA and G. S. SCHULTZ, J. Exp. Med. 163 (1986) 1319

    Article  CAS  Google Scholar 

  6. G. L. BROWN, L. B. NANNEY, J. GRIFFEN, A. B. CRAMER, J. M. YANCEY, I. L. CURTSINGER, L. HOLTZIN, G. S. SCHULTZ, M. J. JURKIEWICZ and J. B. LYNCH, N. Engl. J. Med. 321 (1989) 76

    Article  CAS  Google Scholar 

  7. A. R. C. LEE, Y. SUZUKI, K. H. JUNG, J. NISHIGAKI, Y. HAMAI and A. SHIGEMATSU, Proc. Control. Release Soc. 23 (1996) 325

    Google Scholar 

  8. N. CELEBI, N. ERDEN, B. GONUL and M. KOZ, J. Pharm. Pharmacol. 46 (1994) 386

    CAS  Google Scholar 

  9. B. GONUL, T. SOYLEMEZOGLU, A. BABUL and N. CELEBI, J. Pharm. Pharmacol. 50 (1998) 641

    CAS  Google Scholar 

  10. M. LAATO, J. NIINIKOSKI, L. LEBEL and B. GERDIN, Ann. Surg. 203 (1986) 379

    Article  CAS  Google Scholar 

  11. J. W. THORNTON, C. A. HESS, V. CASSINGHAM and R. H. BARTLETT, Burns. Incl. Therm. Inj. 8 (1982) 156

    CAS  Google Scholar 

  12. H. LI, J. H. SONG, J. S. PARK and K. HAN, Int. J. Pharm. 258 (2003) 11

    Article  CAS  Google Scholar 

  13. V. FALANGA, W. H. EAGLSTEIN, B. BUCALO, M. H. KATZ, B. HARRIS and P. CARSON, J. Dermatol. Surg. Oncol. 18 (1992) 604

    CAS  Google Scholar 

  14. N. CELEBI, A. TURKYILMAZ, B. GONUL and C. OZOGUL, J. Control. Release. 83 (2002) 197

    Article  CAS  Google Scholar 

  15. A. L. RUBIN, T. MIYATA and K. H. STENZEL, J. Macromol. Sci. Chem. 3 (1969) 113

    Article  Google Scholar 

  16. M. CHVAPIL, R. L. KRONENTHAL and W. WINKLE, Connect. Tissue Res. 6 (1973) 1

    CAS  Google Scholar 

  17. M. MAEDA, K. KADOTA, M. KAJIHARA, A. SANO and K. FUJIOKA, J. Control. Release 77 (2001) 261

    Article  CAS  Google Scholar 

  18. T. OKADA, T. HAYASHI and Y. IKADA, Biomaterials 13 (1992) 448

    Article  CAS  Google Scholar 

  19. L. H. H. OLDEDAMINK, P. J. DIJKSTRA, J. A. VANLUYN, P. B. CANWACHEM, P. NIEUWENHUIS and J. FEIJEN, J. Mater. Sci. 6 (1995) 460

    Article  CAS  Google Scholar 

  20. H. O. HO, C. W. LIN and M. T. SHEU, J. Control. Release 77 (2001) 97

    Article  CAS  Google Scholar 

  21. M. T. SHEU, J. C. HUANG, G. C. YEH and H. O. HO, Biomaterials 22 (2001) 1713

    Article  CAS  Google Scholar 

  22. W. A. BUBNIS and C. M. OFNER III, Anal. Biochem. 207 (1992) 129

    Article  CAS  Google Scholar 

  23. X. H. WANG, D. P. LI, W. J. WANG, Q. L. FENG, F. Z. CUI, Y. X. XU, X. H. SONG and M. VAN DER WERF, Biomaterials 24 (2003) 3213

    Article  CAS  Google Scholar 

  24. P. L. RITGER and N. S. PEPPAS, J. Control. Release 5 (1987) 37

    Article  CAS  Google Scholar 

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Acknowledgments

This work was supported by a grant from the National Science Council of Taiwan, R.O.C. (NSC-94-2320-B-214-005). I am indebted to Prof. Yi-Hung Tsai (Kaohsiung Medical University, Taiwan) for his encouragement.

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  1. Department of Biological Science & Technology, I-Shou University, 1, Section 1, Hsueh-Cheng Rd., Ta-Hsu Hsiang, Kaohsiung City 807, Taiwan, R.O.C.

    Chih-Hui Yang

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Yang, CH. Evaluation of the release rate of bioactive recombinant human epidermal growth factor from crosslinking collagen sponges. J Mater Sci: Mater Med 19, 1433–1440 (2008). https://doi.org/10.1007/s10856-007-3249-5

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