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Fabrication and characterization of PCL/gelatin/chitosan ternary nanofibrous composite scaffold for tissue engineering applications

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Abstract

In the present study, we have fabricated a ternary composite nanofibrous scaffold from PCL/gelatin/chitosan, by electrospinning technique, using a solvent system—chloroform/methanol for polycaprolactone (PCL) and acetic acid for gelatin and chitosan, for tissue engineering applications. Field emission scanning electron microscopy (FE-SEM) was used to investigate the fiber morphology of the scaffold and it was found that the fiber morphology was influenced by the concentrations of PCL, gelatin, and chitosan in polymer solution during electrospinning. X-ray diffraction, Fourier transform infrared, and thermogravimetric (TG) analysis results showed some interactions among the molecules of PCL, gelatin, and chitosan within the scaffold. In-vitro cell culture studies were done by seeding L929 mouse fibroblasts on fabricated composite scaffold, which confirmed the cell viability, high cell proliferation rate, and cell adhesion on composite scaffold as indicated by MTT assay, DNA quantification, and FE-SEM analysis of cell-scaffold construct. Thus, the ternary composite scaffold made from the combination of PCL (synthetic polymer), gelatin, and chitosan (natural polymer) may find potential application in tissue engineering.

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Acknowledgements

Chia-Fu Chou and Narayan C. Mishra acknowledge support from the Academia Sinica Nano Program and National Science Council, Taiwan, ROC. (99-2112-M-001-027-MY3) to carry out this research. The authors are thankful to the technical support from Jaslok Hospital and Research Centre, Mumbai, and particularly to Mr. Sachin Ramdas Chaugule for his assistance to carry out biocompatibility study.

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Authors and Affiliations

  1. Department of Polymer and Process Engineering, Indian Institute of Technology Roorkee, Roorkee, India

    Sneh Gautam & Narayan Chandra Mishra

  2. Institute of Physics, Academia Sinica, Taipei, Taiwan

    Chia-Fu Chou

  3. Department of Pathology, All India Institute of Medical Science, New Delhi, New Delhi, India

    Amit Kumar Dinda

  4. Department of Molecular Medicine and Biology, Research Centre, Jaslok Hospital, Mumbai, India

    Pravin D. Potdar

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  1. Sneh Gautam
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  5. Narayan Chandra Mishra
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Correspondence to Narayan Chandra Mishra.

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Gautam, S., Chou, CF., Dinda, A.K. et al. Fabrication and characterization of PCL/gelatin/chitosan ternary nanofibrous composite scaffold for tissue engineering applications. J Mater Sci 49, 1076–1089 (2014). https://doi.org/10.1007/s10853-013-7785-8

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