SpringerLink
Log in

Effect of foliar application with Chlorella vulgaris, Tetradesmus dimorphus, and Arthrospira platensis as biostimulants for common bean

  • Published:
Journal of Applied Phycology Aims and scope Submit manuscript

Abstract

The aim of the present investigation was to study the impact of foliar spraying with Chlorella vulgaris, Arthrospira platensis, and Tetradesmus dimorphus suspensions as biostimulants on growth and yield characteristics of common beans such as Phaseolus vulgaris. Seven treatments were tested during the study: T1 (soil amended with N-urea but no microalgae foliar application), T2 (foliar spraying with C. vulgaris but no N-urea added to the soil), T3 (foliar spraying with T. dimorphus but no N-urea added to the soil), T4 (foliar spraying with A. platensis but no N-urea added to the soil), T5 (foliar spraying with C. vulgaris and soil amended with N-urea), T6 (foliar spraying with T. dimorphus and soil amended with N-urea), T7 (foliar spraying with A. platensis and soil amended with N-urea) and control (untreated). Foliar spraying was applied after 7, 25, and 77 days from sowing using the test microalgae suspensions in concentration of 10 g 100 mL−1. Plant growth and biochemical parameters were measured at the end of both vegetative and fruiting growth stages. Compared with control, the treatments from T1 to T7 showed noticeable increase in all growth parameters and yield attribute. The foliar application with C. vulgaris and chemical fertilizer treated plants (T5) exhibited the maximum increase in total plant height (26.9%), dry weight (37.28%), protein content (48.06 ± 2.403 mg g−1 fresh wt.), and total carbohydrate (394 ± 19.7 mg g−1 dry wt.) during vegetative stage as well as number of pods per plant (5.2 ± 0.26), number of seed/pod (3.5 ± 0.18), pods dry weight (0.95 ± 0.05 g plant−1) during fruiting stage. Thus, it is advisable to use C. vulgaris as a biostimulant for enhancing P. vulgaris growth and crop production.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (Canada)

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

Availability of data

The authors confirm that all the data are available within the article.

References

  • Anagnostidis K, Komárek J (1988) Modern approach to the classification system of cyanophytes. 3-Oscillatoriales. Algol Stud / Arch Hydrobiol Suppl 50–53:327–472

  • Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol 24:1–15

  • Barone V, Puglisi I, Fragalà F, Stevanato P, Baglieri A (2019) Effect of living cells of microalgae or their extracts on soil enzyme activities. Arch Agron Soil Sci 65:712–726

    Article  CAS  Google Scholar 

  • Bayona-Morcillo P, Plaza B, Gómez-Serrano C, Rojas E, Jiménez-Becker S (2020) Effect of the foliar application of cyanobacterial hydrolysate (Arthrospira platensis) on the growth of Petunia x hybrida under salinity conditions. J Appl Phycol 32:4003–4011

    Article  CAS  Google Scholar 

  • Battacharyya D, Babgohari MZ, Rathor P, Prithiviraj B (2015) Seaweed extracts as biostimulants in horticulture. Sci Hort 196:39–48

    Article  CAS  Google Scholar 

  • Bischoff H (1963) Some soil algae from enchanted rock and related algal species. Phycological Studies IV. University of Texas Publ No 6318:1–95

    Google Scholar 

  • Black C (1965) Method of soil analysis part 2. Chem Microbiol Properties 9:1387–1388

    Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  Google Scholar 

  • Bumandalai O, Tserennadmid R (2019) Effect of Chlorella vulgaris as a biofertilizer on germination of tomato and cucumber seeds. Int J Aquat Biol 7:95–99

    Google Scholar 

  • Chiaiese P, Corrado G, Colla G, Kyriacou MC, Rouphael Y (2018) Renewable sources of plant biostimulation: microalgae as a sustainable means to improve crop performance. Front Plant Sci 9:1782

    Article  Google Scholar 

  • Chookalaii H, Riahi H, Shariatmadari Z, Mazarei Z, SeyedHashtroudi M (2020) Enhancement of total flavonoid and phenolic contents in Plantago major L. with plant growth promoting cyanobacteria. J Agric Sci Technol 22:505–518

    Google Scholar 

  • Dias GA, Rocha RHC, Araújo JL, Lima JF, Guedes WA (2016) Growth, yield, and postharvest quality in eggplant produced under different foliar fertilizer (Arthrospira platensis) treatments. Semina Ciênc Agrár 37:3893–3902

    Article  CAS  Google Scholar 

  • Díaz-Batalla L, Widholm JM, Fahey GC, Castaño-Tostado E, Paredes-López O (2006) Chemical components with health implications in wild and cultivated Mexican common bean seeds (Phaseolus vulgaris L.). J Agric Food Chem 54:2045–2052

  • Dineshkumar R, Kumaravel R, Gopalsamy J, Sikder MNA, Sampathkumar P (2018) Microalgae as bio-fertilizers for rice growth and seed yield productivity. Waste Biomass Valor 9:793–800

    Article  CAS  Google Scholar 

  • Dineshkumar R, Subramanian J, Gopalsamy J, Jayasingam P, Arumugam A, Kannadasan S, Sampathkumar P (2019) The impact of using microalgae as biofertilizer in maize (Zea mays L.). Waste Biomass Valor 10:1101–1110

  • Du Jardin P (2015) Plant biostimulants: definition, concept, main categories and regulation. Sci Hort 196:3–14

    Article  Google Scholar 

  • Elarroussia H, Elmernissia N, Benhimaa R, El Kadmiria IM, Bendaou N, Smouni A, Wahbya I (2016) Microalgae polysaccharides a promising plant growth biostimulant. J Algal Biomass Utln 7:55–63

    Google Scholar 

  • Fernández V, Sotiropoulos T, Brown PH (2013) Foliar fertilization: scientific principles and field practices. IFA, Paris pp 12–70

  • Garcia-Gonzalez J, Sommerfeld M (2016) Biofertilizer and biostimulant properties of the microalga Acutodesmus dimorphus. J Appl Phycol 28:1051–1061

    Article  Google Scholar 

  • Gorelova O (2006) Communication of cyanobacteria with plant partners during association formation. Microbiology 75:465–469

    Article  CAS  Google Scholar 

  • Hedge J, Hofreiter B, Whistler R (1962) Carbohydrate chemistry. Academic Press, New York

    Google Scholar 

  • Horvath G, Kissimon J, Faludi-Dániel Á (1972) Effect of light intensity on the formation of carotenoids in normal and mutant maize leaves. Phytochemistry 11:183–187

    Article  CAS  Google Scholar 

  • Kawalekar JS (2013) Role of biofertilizers and biopesticides for sustainable agriculture. J Biol Innov 2:73–78

  • Khan SA, Hussain MZ, Prasad S, Banerjee U (2009) Prospects of biodiesel production from microalgae in India. Renew Sustain Energy Rev 13:2361–2372

    Article  CAS  Google Scholar 

  • Kim MJ, Shim CK, Kim YK, Ko BG, Park JH, Hwang SG, Kim BH (2018) Effect of biostimulator Chlorella fusca on improving growth and qualities of chinese chives and spinach in organic farm. Plant Pathol J 34:567

    Article  CAS  Google Scholar 

  • Kissimon J (1999) Analysis of the photosynthetic pigment composition. International workshop and training course on microalgal biology and biotechnology. Mosonmagyarouar, Hungary, pp 13–26

  • Komárek J, Fott B, Huber-Pestalozzi G (1983) Das Phytoplankton des Süßwassers Systematik und Biologie-Teil 7, 1. Schweizerbart Science Publishers, Stuttgart

  • Lin L-Z, Harnly JM, Pastor-Corrales MS, Luthria DL (2008) The polyphenolic profiles of common bean (Phaseolus vulgaris L.). Food Chem 107:399–410

  • Mógor ÁF, Ördög V, Lima GPP, Molnár Z, Mógor G (2018) Biostimulant properties of cyanobacterial hydrolysate related to polyamines. J Appl Phycol 30:453–460

    Article  Google Scholar 

  • Mourice SK, Tryphone GM (2012) Evaluation of common bean (Phaseolus vulgaris L.) genotypes for adaptation to low phosphorus. Int Schl Res Not 2012:309614

  • Nagy PT, Pintér T (2015) Effects of foliar biofertilizer sprays on nutrient uptake, yield, and quality parameters of Blaufrankish (Vitis vinifera L.) Grapes. Comm Soil Sci Plant Anal 46:219–227

  • Osman MEH, El-Sheekh MM, El-Naggar AH, Gheda SF (2010) Effect of two species of cyanobacteria as biofertilizers on some metabolic activities, growth, and yield of pea plant. Biol Fertil Soils 46:861–875

    Article  Google Scholar 

  • Plaza BM, Gómez-Serrano C, Acién-Fernández FG, Jimenez-Becker S (2018) Effect of microalgae hydrolysate foliar application (Arthrospira platensis and Tetradesmus sp.) on Petunia x hybrida growth. J Appl Phycol 30:2359–2365

    Article  CAS  Google Scholar 

  • Porch TG, Beaver JS, Debouck DG, Jackson SA, Kelly JD, Dempewolf H (2013) Use of wild relatives and closely related species to adapt common bean to climate change. Agronomy 3:433–461

    Article  Google Scholar 

  • Priya TR, Manickavasagan A (2020) Common bean. In: Manickavasagan A, Thirunathan P (eds) Pulses. Springer, Cham, pp 77–97

    Chapter  Google Scholar 

  • Puglisi I, La Bella E, Rovetto EI, Lo Piero AR, Baglieri A (2020) Biostimulant effect and biochemical response in lettuce seedlings treated with a Tetradesmus quadricauda extract. Plants 9:123

    Article  CAS  Google Scholar 

  • Raschke RL, Schultz DA (1987) The use of the algal growth potential test for data assessment. J Water Pollut Control Fed 222–227

  • Ronga D, Biazzi E, Parati K, Carminati D, Carminati E, Tava A (2019) Microalgal biostimulants and biofertilisers in crop productions. Agronomy 9:192

    Article  CAS  Google Scholar 

  • Sadasivam S, Manickam A (1996) Biochemical methods. New Age International (P) Limited, New Delhi 2:124–126

  • Shaaban MM (2001a) Green microalgae water extract as foliar feeding to wheat plants. Pak J Biol Sci 4:628–632

    Article  Google Scholar 

  • Shaaban MM (2001b) Nutritional status and growth of maize plants as affected by green microalgae as soil additives. J Biol Sci 1:475–479

    Article  Google Scholar 

  • Shindy WW, Smith OE (1975) Identification of plant hormones from cotton ovules. Plant Physiol 55:550–554

    Article  CAS  Google Scholar 

  • Siddiq M, Ravi R, Harte J, Dolan K (2010) Physical and functional characteristics of selected dry bean (Phaseolus vulgaris L.) flours. LWT - Food Sci Technol 43:232–237

  • Starr RC (1978) The culture collection of algae at the University of Texas at Austin. J Phycol 14:47–100

  • Stein JR, Hellebust JA, Craigie J (1973) Handbook of phycological methods: culture methods and growth measurements. Cambridge University Press, Cambridge

  • Stoscheck CM (1990) Quantitation of protein. Meth Enzymol 182:50–68

  • Tarakhovskaya E, Maslov YI, Shishova M (2007) Phytohormones in algae. Russ J Plant Physiol 54:163–170

    Article  CAS  Google Scholar 

  • Van Handel E (1968) Direct micro determination of sucrose. Anal Biochem 22:280–283

    Article  Google Scholar 

  • Yemm E, Willis A (1954) The estimation of carbohydrates in plant extracts by anthrone. Biochem J 57:508–514

    Article  CAS  Google Scholar 

  • Zarrouk C (1966) Contribution a l'etude d'une Cyanophycee. Influence de Divers Facteurs Physiques et Chimiques sur la croissance et la photosynthese de Spirulina maxima. Thesis. University of Paris, France

Download references

Acknowledgements

The authors would like to express their gratitude to the Botany Department at Mansoura University in Egypt for allowing them to complete these experiments and for providing financial support.

Author information

Authors and Affiliations

  1. Botany Department, Faculty of Science, Mansoura University, Mansoura, 35516, Egypt

    Dina A. Refaay, Eman M. El-Marzoki, Mohammed I. Abdel-Hamid & Samia A. Haroun

Authors
  1. Dina A. Refaay
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Eman M. El-Marzoki
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mohammed I. Abdel-Hamid
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Samia A. Haroun
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

D.A.R. prepared the manuscript. D.A.R., E.M.E., M.I.A, and S.A.H conducted the experiments. S.A.H and D.A.R. designed the study. All authors have read and approved the final manuscript.

Corresponding author

Correspondence to Dina A. Refaay.

Ethics declarations

Conflict of interest

The authors declare no competing interests.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Refaay, D.A., El-Marzoki, E.M., Abdel-Hamid, M.I. et al. Effect of foliar application with Chlorella vulgaris, Tetradesmus dimorphus, and Arthrospira platensis as biostimulants for common bean. J Appl Phycol 33, 3807–3815 (2021). https://doi.org/10.1007/s10811-021-02584-z

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10811-021-02584-z

Keywords

Search

Navigation