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The Functional Interaction Between CDK11p58 and β-1,4-Galactosyltransferase I Involved in Astrocyte Activation Caused by Lipopolysaccharide

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Abstract

Glial cells are mediating the main activation of the central nervous system (CNS), being astrocytes the mayor glial cells in the brain. Glial activation may result beneficial since it could promote tissue repair and pathogen elimination. However, excessive glial activation mechanism can also have do harm to the tissue. β-1,4-Galactosyltransferase I (β-1,4-GalT-I) is a key inflammatory mediator that participates in the initiation and maintenance of inflammatory reaction in some diseases. Moreover, CDK11p58 has been reported to be associated with β-1,4-GalT-I. We have found that CDK11p58 and β-1,4-GalT-I are induced in lipopolysaccharide (LPS)-challenged rat primary astrocytes in a affinis dose- and time-dependent manner. CDK11p58 regulates the expression of β-1,4-GalT-I by interacting with it. After the knockdown of CDK11p58 expression, the expression of β-1,4-GalT-I decreases, and astrocyte activation downregulates. Inversely, the expression of β-1,4-GalT-I increases, and astrocyte activation enhances due to the overexpression of CDK11p58. Knockdown of β-1,4-GalT-I reduces the activation potentiation caused by the overexpression of CDK11p58, illustrating the function of CDK11p58 to promote astrocyte activation depends on β-1,4-GalT-I. The interaction between CDK11p58 and β-1,4-GalT-I to upregulate astrocyte activation is related to activating p38 and JNK pathways. These findings indicated that the functional interaction between CDK11p58 and β-1,4-GalT-I may play an important role during astrocyte activation after LPS administration.

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Acknowledgements

This work was supported by the National Basic Research Program of China (973 Program, No.2011CB910604, and No.2012BC822104); the National Natural Science Foundation of China (No.31070723, No.81070275 and No. 81172879); Natural Science Foundation of Jiangsu province (No.BK2009157, and No.BK2010169); Key Project Natural Science Foundation of Jiangsu University and College (No.11KJA310002); A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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Authors and Affiliations

  1. Department of Pathogenbiology, Medical College, Nantong University, Nantong, 226001, Jiangsu, People’s Republic of China

    **aojuan Liu

  2. Department of Immunology, Medical College, Nantong University, Nantong, 226001, Jiangsu, People’s Republic of China

    Chun Cheng, Bai Shao, **aohong Wu, Yuhong Ji & Aiguo Shen

  3. Department of Geriatics, The Second Affiliated Hospital of Nan**g Medical University, Nan**g, 210011, Jiangsu, China

    **ang Lu

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Correspondence to **ang Lu or Aiguo Shen.

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Liu, X., Cheng, C., Shao, B. et al. The Functional Interaction Between CDK11p58 and β-1,4-Galactosyltransferase I Involved in Astrocyte Activation Caused by Lipopolysaccharide. Inflammation 35, 1365–1377 (2012). https://doi.org/10.1007/s10753-012-9450-9

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