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Semaphorin 3A promotes osteogenic differentiation of BMSC from type 2 diabetes mellitus rats

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Abstract

Bone regeneration is impaired in patients with type 2 diabetes mellitus (T2DM), which leads to non-healing after bone loss. The decreased osteogenic capacity of bone mesenchymal stem cells (BMSCs) might be a main reason. Sema3A, as a powerful protein promoting osteocyte differentiation, shows potential for bone regeneration treatment. BMSCs may be a therapeutic solution. In this study, we divided BMSCs from T2DM rats (BMSCs-D) and normal rats (BMSCs-N), identified their ability to differentiate into different cell types. Then we found decreased expression of Sema3A in BMSCs-D compared with BMSCs-N. Stimulating with Sema3A showed no influence in the proliferation or migration of BMSCs. However, Sema3A stimulation significantly increased the expression of osteogenic‑related genes, including type I collagen, alkaline phosphatase, Runt-related transcription factor 2 (RUNX2), bone morphogenetic protein and osteocalcin. Besides, the osteogenic capacity of BMSCs was also increased by Sema3A stimulation. In conclusion, we proved that exogenous Sema3A stimulation might repair the osteogenic capacity of BMSCs-D, thus providing a new strategy for restoring the impaired bone regeneration ability for T2DM patients.

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Acknowledgements

This study is supported by the National Natural Science Foundation of China [NO. 81470775]. Department of Burns and Cutaneous Surgery provided most of the equipment of experiment.

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Authors and Affiliations

  1. School of Stomatology, Shanxi Medical University, 56 **njian South Road, Taiyuan, 030012, China

    Qiao Qiao & Fenglan Li

  2. Department of Implantation, School of Stomatology, The Fourth Military Medical University, 145 Changle West Road, **’an, 710032, China

    **aoru Xu & Yingliang Song

  3. Peking University Health Science Center, 38 Xueyuan Street, Bei**g, 100191, China

    Shuang Song

  4. Department of Stomatology, Shaanxi Second Provincial People’s Hospital, 3 Shangqin Road, **’an, 710005, China

    Wenzhong Zhu

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  1. Qiao Qiao
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  2. **aoru Xu
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  3. Yingliang Song
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  4. Shuang Song
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  5. Wenzhong Zhu
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  6. Fenglan Li
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Correspondence to Fenglan Li.

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Qiao, Q., Xu, X., Song, Y. et al. Semaphorin 3A promotes osteogenic differentiation of BMSC from type 2 diabetes mellitus rats. J Mol Hist 49, 369–376 (2018). https://doi.org/10.1007/s10735-018-9776-1

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  • DOI: https://doi.org/10.1007/s10735-018-9776-1

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