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QTL map** of lentil anthracnose (Colletotrichum lentis) resistance from Lens ervoides accession IG 72815 in an interspecific RIL population

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Abstract

Anthracnose, caused by Colletotrichum lentis, is one of the most damaging diseases of lentil (L. culinaris) in western Canada. Lens ervoides accession IG 72815 exhibits high levels of resistance to the pathogenic races 0 and 1. The objective of this study was to identify quantitative trait loci (QTL) associated with anthracnose resistance in lentil using a recombinant inbred line (RIL) population from the interspecific cross between IG 72815 and the susceptible cultivar Eston. A total of 168 RILs were genotyped and evaluated for anthracnose race 0 and race 1 reaction in the growth chamber and polyhouse. QTL analysis identified major resistance loci on chromosomes 3 and 7 for both races, accounting for 20.1–31.2% and 8.3–18.4%, respectively, of the total phenotypic variation for anthracnose resistance. Multiple classes of putative defense-related genes are located within both loci. Further characterization of these regions will facilitate the introgression of anthracnose resistance from Lens ervoides into elite lentil cultivars via marker-assisted selection.

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Data availability

The raw genotypic data supporting this study are available at https:// knowpulse.usask.ca/ Geneticmap/2691115 upon request from the authors.

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Acknowledgements

The authors gratefully acknowledge funding from the Natural Sciences and Engineering Research Council of Canada (NSERC) Industrial Research Chair Program, the Saskatchewan Pulse Growers, and the University of Saskatchewan. We are also thankful for the technical assistance of the bioinformatics, molecular and pathology lab staff of the Pulse Breeding and Genetics group at the University of Saskatchewan.

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Authors and Affiliations

  1. Crop Development Centre, Department of Plant Sciences, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK, S7N 5A8, Canada

    Tadesse S. Gela, Chu Shin Koh, Carolyn T. Caron, Li-An Chen, Albert Vandenberg & Kirstin E. Bett

  2. Global Institute for Food Security, University of Saskatchewan, 421 Downey Road, Saskatoon, SK, S7N 4L8, Canada

    Chu Shin Koh

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  1. Tadesse S. Gela
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  2. Chu Shin Koh
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Contributions

TSG generated phenotypic data, preformed data analyses and wrote the manuscript; LC performed the genoty** of the map** population; CC carried out SNP calling; CSK constructed the genetic map, AV and KEB conceived the experiment and critically reviewed the manuscript. All authors read and approved the final version.

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Correspondence to Kirstin E. Bett.

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Gela, T.S., Koh, C.S., Caron, C.T. et al. QTL map** of lentil anthracnose (Colletotrichum lentis) resistance from Lens ervoides accession IG 72815 in an interspecific RIL population. Euphytica 217, 70 (2021). https://doi.org/10.1007/s10681-021-02804-0

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