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Genetic analysis and molecular map** of QTLs associated with resistance to bacterial blight in a rice mutant, SA0423

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Abstract

Bacterial blight disease, caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the most serious diseases in rice producing areas. SA0423 is a broad-range resistance mutant selected from a popular japonica-type variety, TNG67, using sodium azide mutagenesis. Genetic analysis and QTL map** of SA0423 were performed using the descendants obtained from crossing with Taichung Native 1, a susceptible and well-known indica variety, by challenging with a Taiwanese Xoo isolate, XF89b. Genetic analysis displayed that the resistance of SA0423 is regulated by quantitative trait loci (QTLs) with incomplete dominance. A linkage map covering 12 chromosomes and consisting of 148 SSR as well as 3 InDel markers was constructed. Three QTLs are identified on chromosomes 11, 8 and 6 and account for 21.1, 11 and 9.6 % of the observed phenotypic variance, respectively. Three QTLs are localized to 6, 7 and 14 confidence intervals, respectively. These QTLs contribute to approximately 47 % of the total phenotypic variation of the F2 population. No epistatic effect could be detected among the three QTLs. Our results provide a suitable source of potential disease resistance genes and establish a system for improving rice bacterial blight resistance through marker-assisted selection.

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Acknowledgments

The financial support from the Ministry of Science and Technology (NSC 102-2317-B-005-01) of Taiwan to C. S. Wang is acknowledged. Suggestions from anonymous reviewers that improved the quality of this article were highly appreciated.

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    Authors and Affiliations

    1. Biotechnology Division, Taiwan Agricultural Research Institute, 189, Chung-Cheng Rd., Wufeng, Taichung, 41362, Taiwan, ROC

      Hsin-Yi Tseng, Da-Gin Lin, Hsiao-Ying Hsieh & Ya-June Tseng

    2. Department of Agronomy, National Chung-Hsing University, No. 250 Kuo-Kuang Rd., Taichung, 40227, Taiwan, ROC

      Ya-June Tseng, Wen-Bin Tseng & Chang-Sheng Wang

    3. Plant Pathology Division, Taiwan Agricultural Research Institute, 189, Chung-Cheng Rd.,Wufeng, Taichung, 41362, Taiwan, ROC

      Chun-Wei Chen

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    1. Hsin-Yi Tseng
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    Hsin-Yi Tseng and Da-Gin Lin have contributed equally to this manuscript.

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    Tseng, HY., Lin, DG., Hsieh, HY. et al. Genetic analysis and molecular map** of QTLs associated with resistance to bacterial blight in a rice mutant, SA0423. Euphytica 205, 231–241 (2015). https://doi.org/10.1007/s10681-015-1435-y

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