SpringerLink
Log in

Ploidy manipulation and introgression breeding in Darwin hybrid tulips

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

Meiotic polyploidisation via crossing with 2n gamete producing genotypes and interploidy crosses are two of the main methods currently used to obtain polyploid tulips. In our study diploid 2n gamete producing F1 hybrids of Darwin hybrids (Tulipa gesneriana × Tulipa fosteriana) and triploid hybrid resulting from ‘Rhodos’ × ‘Princeps’ cross were used as pollen donor and crossed with cultivars of T. gesneriana in the following combination: 2x × 2x, 3x × 2x, 2x × 3x, and 3x × 3x. The progenies resulting from crosses at diploid level were mostly diploid, whereas a few seedlings were triploid. In 3x × 2x crosses aneuploids with chromosome constitution in between triploid and tetraploid (43–45 chromosomes) were predominant, but also one tetraploid (2n = 4x = 48) and four pentaploids (2n = 5x = 60) were obtained. In 2x × 3x crosses most progenies were triploid with the exception of a few aneuploids (3x + 1 and 3x − 1), whereas in 3x × 3x cross diploid and aneuploid genotypes were recorded with chromosome number varied from 27 to 34. These results indicate that triploid parents produced aneuploid as well as euploid (x, 2x, 3x) gametes and that success in ploidy manipulation in tulip depends to a large degree on the ploidy level of the parental genotypes used for hybridization. Genome constitution of selected population of F1 and BC1 hybrids was analyzed through genomic in situ hybridization (GISH). GISH analysis of the BC1 showed a considerable amount of intergenomic recombination which is desirable for introgression breeding.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (Germany)

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Bamford R, Reynard GB, Bellows JM Jr (1939) Chromosome number in some tulip hybrids. Bot Gaz 101:482–490

    Article  Google Scholar 

  • Barba-Gonzalez R, Lokker AC, Lim KB, Ramanna MS, Van Tuyl JM (2004) Use of 2n gametes for the production of sexual polyploids from sterile Oriental × Asiatic hybrids of lilies (Lilium). Theor Appl Genet 109:1125–1132

    Article  CAS  PubMed  Google Scholar 

  • Barba-Gonzalez R, Lim KB, Ramanna MS, Visser RGF, Van Tuyl JM (2005) Occurrence of 2n gametes in the F1 hybrids of Oriental × Asiatic lilies (Lilium): relevance to intergenomic recombination and backcrossing. Euphytica 143:67–73

    Article  CAS  Google Scholar 

  • Barba-Gonzalez R, Miller CT, Ramanna MS, Van Tuyl JM (2006a) Induction of 2n gametes for overcoming F1-sterility in lily and tulip. Acta Hort 714:99–106

    Google Scholar 

  • Barba-Gonzalez R, Van Silfhout A, Visser RGF, Ramanna MR, Van Tuyl JM (2006b) Progenies of allotetraploids of Oriental × Asiatic lilies (Lilium) examined by GISH analysis. Euphytica 151:243–250

    Article  Google Scholar 

  • Barba-Gonzalez R, Lim KB, Zhou S, Ramanna MS, Van Tuyl JM (2008) Interspecific hybridization in Lily: the use of 2n gametes in interspecific lily hybrids. In: Allen L (ed) Floriculture, ornamental plant breeding, 5th edn. Global Science Books, London

  • Brandham PE (1982) Inter-embryo competition in the progeny of autotriploid Aloineae (Liliaceae). Genetica 59:29–42

    Article  Google Scholar 

  • Chauvin JE, Label A, Kermarrec MP (2006) In vitro chromosome-doubling in tulip (Tulipa gesneriana L.). J Hortic Sci Biotechnol 80:693–698

    Google Scholar 

  • Chen C, Lyon MT, O’Malley D, Federici CT, Gmitter J, Grosser JW, Chaparro JX, Roose ML, Gmitter FG Jr (2008) Origin and frequency of 2n gametes in Citrus sinensis × Poncirus trifoliate and their reciprocal crosses. Plant Sci 174:1–8

  • Eikelboom W, Straathof ThP, Van Tuyl JM (2001) Tetraploide “Christmas Marvel” methoden om tetrapoide tulpen te verkrijgen. Bloembollencultuur 112:22–23

    Google Scholar 

  • Fulton ThM, Chunzoongse J, Tanksley SD (1995) Micropreparation protocol for extraction of DNA from tomato and other Herbaceous plants. Plant Mol Biol Rep 13:207–209

    Article  CAS  Google Scholar 

  • Holitscher O (1968) Pruhonicky sortiment tulipanu. Acta Pruh 18:1–215

    Google Scholar 

  • Johnston SA, Den Nijs TMP, Peloquin SJ, Hanneman RE Jr (1980) The significance of genic balance to endosperm development in intraspecific crosses. Theor Appl Genet 57:5–9

    CAS  PubMed  Google Scholar 

  • Khan N, Barba-Gonzalez R, Ramanna MS, Visser RGF, Van Tuyl JM (2009) Construction of chromosomal recombination maps of three genomes of lilies (Lilium) based on GISH analysis. Genome 52:238–251

    Article  CAS  PubMed  Google Scholar 

  • Khan N, Barba-Gonzalez R, Ramanna MS, Arens P, Visser RGF, Van Tuyl JM (2010) Relevance of unilateral and bilateral sexual polyploidisation in relation to intergenomic recombination and introgression in Lilium species hybrids. Euphytica 171:157–173

    Article  Google Scholar 

  • Kroon GH (1975) Chromosome numbers of garden tulips. Acta Bot Neerl 24:489–490

    Google Scholar 

  • Kroon GH, Jongerius MC (1986) Chromosome numbers of Tulipa species and the occurrence of hexaploidy. Euphytica 35:73–76

    Article  Google Scholar 

  • Kroon GH, Van Eijk JP (1977) Polyploidy in tulips (Tulipa L.). The occurrence of diploid gametes. Euphytica 26:63–66

    Article  Google Scholar 

  • Lim KB, Ramanna MS, De Jong JH, Jacobsen E, Van Tuyl JM (2001) Indeterminate meiotic restitution (IMR): a novel type of meiotic nuclear restitution mechanism detected in interspecific lily hybrids by GISH. Theor Appl Genet 103:219–230

    Article  CAS  Google Scholar 

  • Lim KB, Ramanna MS, Jacobsen E, Van Tuyl JM (2003) Evaluation of BC2 progenies derived from 3x − 2x and 3x − 4x crosses of Lilium hybrids: a GISH analysis. Theor Appl Genet 106:568–574

    PubMed  Google Scholar 

  • Lim KB, Shen TM, Barba-Gonzalez R, Ramanna MS, Van Tuyl JM (2004) Occurrence of SDR 2n-gametes in Lilium hybrids. Breed Sci 54:13–18

    Article  Google Scholar 

  • Marasek A, Okazaki K (2007) GISH analysis of hybrids produced by interspecific hybridization between T. gesneriana and T. fosteriana. Acta Hort 743:133–137

    CAS  Google Scholar 

  • Marasek A, Okazaki K (2008) Analysis of introgression of the Tulipa fosteriana genome into Tulipa gesneriana using GISH and FISH. Euphytica 160:217–230

    Article  CAS  Google Scholar 

  • Marasek A, Mizuochi H, Okazaki K (2006) The origin of Darwin hybrid tulips analyzed by flow cytometry. Karyotype analyses and genomic in situ hybridization. Euphytica 151:279–290

    Article  Google Scholar 

  • Marasek-Ciolakowska A, Ramanna MS, Van Tuyl JM (2009) Introgression breeding in genus Tulipa analysed by GISH. Acta Hort 836:105–110

    Google Scholar 

  • Marasek-Ciolakowska A, Ramanna MS, Van Tuyl JM (2011) Introgression of chromosome segments of T. fosteriana into T. gesneriana detected through GISH and its implications for breeding virus resistance tulips. Acta Hort 885:175–182

    Google Scholar 

  • Marasek-Ciolakowska A, He H, Bijman P, Ramanna MS, Arens P, Van Tuyl JM (2012a) Assessment of intergenomic recombination through GISH analysis of F1, BC1 and BC2 progenies of Tulipa gesneriana and Tulipa fosteriana. Plant Syst Evol 298:887–899

    Article  CAS  Google Scholar 

  • Marasek-Ciolakowska A, **e S, Ramanna MS, Arens P, Van Tuyl JM (2012b) Meiotic polyploidisation in Darwin hybrid tulips. Acta Hort 953:187–192

    Google Scholar 

  • Mizuochi H, Matsuzaki H, Moue T, Okazaki K (2009) Diploid endosperm formation in Tulipa spp. And identification of a 1:1 maternal to paternal genome ratio in endosperm of Tulipa gesneriana L. Sex Plant Rep 1:27–36. doi:10.1007/s00497-008-0088-6

    Article  Google Scholar 

  • Okazaki K (2005) New aspects of tulip breeding: embryo culture and polyploidy. Acta Hort 673:127–140

    Google Scholar 

  • Okazaki K, Nishimura M (2000) Ploidy of progenies crossed between diploids, triploids and tetraploids in tulip. Acta Hort 522:127–134

    Google Scholar 

  • Okazaki K, Kurimoto K, Miyajima I, Enami A, Mizuochi H, Matsumoto Y, Ohya H (2005) Induction of 2n pollen in tulips by arresting the meiotic process with nitrous oxide gas. Euphytica 143:101–114

    Article  CAS  Google Scholar 

  • Plavcová O, Holitscher D, Petrova E (1976) Dinitrogen-oxide induction of polyploidy in Tulip. Sbornik Uvtiz-Zahradni Ctvi 3:207–216

    Google Scholar 

  • Ramanna MS, Jacobsen E (2003) Relevance of sexual polyploidisation for crop improvement—a review. Euphytica 133:3–18

    Article  Google Scholar 

  • Ramanna MS, Kuipers AGJ, Jacobsen E (2003) Occurrence of numerically unreduced (2n) gametes in Alstroemeria interspecific hybrids and their significance for sexual polyploidisation. Euphytica 133:95–106

    Article  Google Scholar 

  • Ramanna MS, Marasek-Ciolakowska A, **e S, Khan N, Van Tuyl JM (2012) The significance of polyploidy for Bulbous ornamentals: a molecular cytogenetic assessment. In: Van Jaap Tuyl, Paul Arens (eds) Floriculture and ornamental biotechnology, 6th edn. Global Science Books, London, pp 116–121

  • Stephens LC (1998) Formation of unreduced pollen by an impatiens Hawkeri × Platypetala interspecific hybrid. Hereditas 128:251–255

    Article  Google Scholar 

  • Straathof ThP, Eikelboom W (1997) Tulip breeding at PRI. Daffodil Tulip Yearbook 8:27–33

    Google Scholar 

  • Upcott M, Philip J (1939) The genetic structure of Tulipa IV. Balance, selection and fertility. J Genetics 38:91–123

    Article  Google Scholar 

  • Van Laere K, Dewitte A, Van Huylenbroeck J, Van Bockstaele E (2009) Evidence for the occurrence of unreduced gametes in interspecific hybrids of Hibiscus. J Hort Sci Biotech 84:240–247

    Google Scholar 

  • Van Scheepen J (1996) Classified list and international register of Tulip names. Royal General Bulbgrowers’ Association KAVB, Hillegom

    Google Scholar 

  • Van Tuyl JM, Boon E (1997) Variation in DNA-content in the genus Lilium. Acta Hort 430:829–835

    Google Scholar 

  • Van Tuyl JM, Van Creij MGM (2007) Tulipa gesneriana and Tulip hybrids. In: Anderson NO (ed) Flower breeding and genetics. Springer, Oklahoma, pp 623–641

    Google Scholar 

  • Van Tuyl JM, Meijer B, Van Diën MP (1992) The use of oryzalin as an alternative for colchicine in in vitro chromosome doubling of Lilium and Nerine. Acta Hort 352:625–630

    Google Scholar 

  • Van Tuyl JM, Lim KB, Ramanna MS (2002) Interspecific hybridization and introgression. In: Vainstein A (ed) Breeding for ornamentals: classical and molecular approaches. Kluwer Academic Publishers, Dordrecht, pp 85–103

    Chapter  Google Scholar 

  • **e S, Khan N, Ramanna MS, Niu L, Marasek-Ciolakowska A, Arens P, Van Tuyl JM (2010) An assessment of chromosomal rearrangements in neopolyploids of Lilium hybrids. Genome 53:439–446

    Article  PubMed  Google Scholar 

  • Zeilinga AE, Schouten HP (1968a) Polyploidy in garden Tulips. I. Survey of Tulipa varieties for polyploids. Euphytica 17:252–264

    Google Scholar 

  • Zeilinga AE, Schouten HP (1968b) Polyploidy in garden Tulips. II. The production of tetraploids. Euphytica 17:303–310

    Google Scholar 

  • Zhong C, Zhang S-n Yu, X-h Li Y, X-l Hou, Li S-j (2010) Studies on the induction of 2n gametes in Chinese cabbage and the production of tetraploid by sexual polyploidisation. Acta Hort Sinica 37:1789–1795

    Google Scholar 

  • Zhou S, Ramanna MS, Visser RGF, Van Tuyl JM (2008) Genome composition of triploid lily cultivars derived from sexual polyploidization of Longiflourm × Asiatic hybrids (Lilium). Euphytica 160:207–215

    Article  CAS  Google Scholar 

  • Zwierzykowski Z, Tayyar R, Brunell M, Lukaszewski AJ (1998) Genome recombination in intergeneric hybrids between tetraploid Festuca pratensis and Lolium multiflorum. J Heredity 89:324–328

    Google Scholar 

Download references

Acknowledgments

The authors wish to thank Prof. Keiichi Okazaki (Niigata University, Faculty of Agriculture, Niigata, Japan) for his valuable comments on the manuscript.

Author information

Authors and Affiliations

  1. Laboratory of Biochemistry and Molecular Biology, Department of General and Molecular Biology, Research Institute of Horticulture, Konstytucji 3 Maja Str. 1/3, 96-100, Skierniewice, Poland

    Agnieszka Marasek-Ciolakowska

  2. Sino-Europe Agricultural Development Centre (SEADC), Wageningen Road No. 1, Zhangzhou Merchant Development Zone, Zhangzhou, 363105, Fujian, People’s Republic of China

    Songlin **e

  3. Plant Breeding, Wageningen University and Research Centre, P.O. Box 386, 6700 AJ, Wageningen, The Netherlands

    Paul Arens & Jaap M. van Tuyl

Authors
  1. Agnieszka Marasek-Ciolakowska
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Songlin **e
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Paul Arens
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jaap M. van Tuyl
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Agnieszka Marasek-Ciolakowska.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Marasek-Ciolakowska, A., **e, S., Arens, P. et al. Ploidy manipulation and introgression breeding in Darwin hybrid tulips. Euphytica 198, 389–400 (2014). https://doi.org/10.1007/s10681-014-1115-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10681-014-1115-3

Keywords

Search

Navigation