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Genomic characterization of drought tolerance-related traits in spring wheat

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Drought tolerance was investigated in ‘C306’, one of the most drought tolerant wheat cultivars bred in India in the 1960’s. An intervarietal map** population of recombinant inbred lines of the cross ‘C306’ × ‘HUW206’ was evaluated for drought tolerance components, namely potential quantum efficiency of photosystem (PS) II (Fv/Fm), chlorophyll content (Chl), flag leaf temperature (Lt), and grain yield per plant (Gyp) under stress. Three independent experiments were conducted under well-watered and water-stressed conditions in greenhouses and growth chambers at Kansas State University (USA). Five hundred and sixty microsatellite markers covering the entire genome were screened for polymorphism between the parents. A QTL (QLt.ksu-1D) for Lt (low flag leaf temperature under stress) on the short arm of chromosome 1D between markers Xbarc271 and Xgwm337 at LOD 3.5 explained 37% of the phenotypic variation. A QTL for Fv/Fm (QF v /F m .ksu-3B) and Chl (QChl.ksu-3B) controlling quantum efficiency of PS II and chlorophyll content under stress were co-localized on chromosome 3B in the marker interval Xbarc68–Xbarc101 and explained 35–40% of the phenotypic variation for each trait. A QTL (QGyp.ksu-4A) for Gyp on chromosome 4A at a LOD value of 3.2 explained 16.3% of the phenotypic variation. Inconsistent QTLs were observed for Fv/Fm on chromosomes 3A, 6A, 2B, 4B, and 4D; for Chl on 3A, 6A, 2B and 4B; and for Lt on 1A, 3A 6A, 3B and 5B. The identified QTLs give a first glimpse of the genetics of drought tolerance in C306 and need to be validated in field experiments using the marker-phenotype linkages reported here.

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Acknowledgments

This research was supported by the Ministry of Science and Technology, Government of India, Delhi, through a Better Opportunities for Young Scientists in Chosen Areas of Science and Technology (BOYSCAST) Fellowship to Dr. Sundeep Kumar for doing advanced research at the Wheat Genetic and Genomic Resources Center, Kansas State University. We also acknowledge a Coordinated Agricultural Project Grant (no. 2011–68002–30029, Triticeae-CAP) from the USDA National Institute of Food and Agriculture for partial financial support. We thank Duane Wilson and Jon Raupp for excellent technical assistance. This is contribution no. 12-256-J from the Kansas Agricultural Experiment Station, Manhattan, KS 66506-5502.

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Authors and Affiliations

  1. Wheat Genetic and Genomic Resources Center, Department of Plant Pathology, Kansas State University, Manhattan, KS, 66506, USA

    Sundeep Kumar, Sunish Kumar Sehgal & Bikram Singh Gill

  2. NRC on DNA Fingerprinting, National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi, 110012, India

    Sundeep Kumar

  3. The Energy and Resources Institute (TERI), IHC Complex, Lodhi Road, New Delhi, 110003, India

    Uttam Kumar

  4. Department of Agronomy, Kansas State University, Manhattan, KS, 66506, USA

    P. V. Vara Prasad

  5. CIMMYT South Asia, Singha Durbar Road, Kathmandu, Nepal

    Arun Kumar Joshi

  6. Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, India

    Arun Kumar Joshi

  7. Department of Biological Sciences, Faculty of Science, Genomics and Biotechnology Section, King Abdulaziz University, Jeddah, 21589, Saudi Arabia

    Bikram Singh Gill

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  1. Sundeep Kumar
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Correspondence to Arun Kumar Joshi.

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Kumar, S., Sehgal, S.K., Kumar, U. et al. Genomic characterization of drought tolerance-related traits in spring wheat. Euphytica 186, 265–276 (2012). https://doi.org/10.1007/s10681-012-0675-3

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