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Screening of wild crucifers for resistance to mustard aphid, Lipaphis erysimi (Kaltenbach) and attempt at introgression of resistance gene(s) from Brassica fruticulosa to Brassica juncea

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Abstract

A diverse array of wild and weedy crucifers was screened under laboratory conditions for their resistance to Lipaphis erysimi (Kaltenbach) (Homoptera: Aphididae). Among these, Brassica fruticulosa and Brassica montana were found to be the most promising. The availability of a synthetic amphiploid, AD-4(B. fruticulosa × Brassica rapa var. brown sarson) as well as a set of Brassica juncea lines carrying genomic introgressions from B. fruticulosa allowed us to investigate B. fruticulosa resistance in greater detail. This assessment was carried out along with susceptible check B. rapa ssp. brown sarson cv. BSH-1 in a series of choice and no choice experiments. The mustard aphid showed maximum preference for feeding on BSH-1 while least preference was recorded for B. fruticulosa followed by AD-4 as evidenced by the number of aphids that settled on circular leaf bits of these genotypes 24 and 48 h after release in a choice experiment. Brassica fruticulosa exhibited strong antibiosis against mustard aphid in no choice experiment and all the released aphids died within 5–8 days of their release, while the maximum survival (76.7%) was recorded on BSH-1. The survival on AD-4 (40%) was significantly lower than that on BSH-1. Almost similar trend was observed with respect to other demographic parameters of L. erysimi viz. development time, fecundity and longevity. In the screen house studies, there was no seedling mortality in B. fruticulosa and AD-4 after 30 days of aphid release while 80% mortality was observed on BSH-1. Excellent variation for aphid resistance was recorded in B. juncea introgression lines, emphasizing heritable nature of fruticulosa resistance. The biochemical analysis suggested the possibility of high concentration of lectins to be associated with low aphid infestation in B. fruticulosa.

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Acknowledgments

First and second authors contributed equally to the research. The studies were partly funded under ICAR National Professor Project “Broadening the genetic base of Indian mustard (Brassica juncea) through alien introgressions and germplasm enhancement” awarded to Surinder S. Banga.

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Authors and Affiliations

  1. Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, 141004, Punjab, India

    Sarwan Kumar, Chhaya Atri, Manjeet K. Sangha & S. S. Banga

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  1. Sarwan Kumar
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  2. Chhaya Atri
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  4. S. S. Banga
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Correspondence to S. S. Banga.

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Kumar, S., Atri, C., Sangha, M.K. et al. Screening of wild crucifers for resistance to mustard aphid, Lipaphis erysimi (Kaltenbach) and attempt at introgression of resistance gene(s) from Brassica fruticulosa to Brassica juncea . Euphytica 179, 461–470 (2011). https://doi.org/10.1007/s10681-011-0351-z

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