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Leaf and neck blast resistance reaction in tropical rice lines under green house condition

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Rice blast (Magnaporthe oryzae) is the most destructive and epidemic disease of rice. Use of host resistance is the best alternative for disease management. The leaf and neck blast resistance of 182 rice breeding lines were assessed for leaf and neck blast and classified relative to a susceptible check-Masuli and resistant check-Laxmi, from greenhouse experiment in 2005 and 2006. The test plants were inoculated with 105 conidial suspension/ml of M. oryzae at 21 days old seedlings for the leaf blast, and at neck base for the neck blast. Among them, for leaf blast, 77 rice lines were resistant, 43 were moderately resistant, 39 were moderately susceptible and 23 were susceptible. While among the selected 31 rice lines evaluated for neck blast, 4 lines were resistant, 4 were moderately resistant, 16 were moderately susceptible and 7 were susceptible. Leaf and neck infection was significant and positively correlated (r = 0.30, P = 0.05). The rice lines, Barkhe 1032, Barkhe 1034, Barkhe 1035, Barkhe 1036, Barkhe 2014, Barkhe 2024, Barkhe 3019, Super 3004 were resistant to leaf blast and Barkhe 1006, Barkhe 1032, Barkhe 1035, Barkhe 3004 were resistant to neck blast. The rice lines with identification # 11, 69, 137, 168, 182 from Masuli × MT4 parentage, and Barkhe 3017 were susceptible to both leaf and neck blast. Progenies of Irradiated Pusa Basmati (IPB), Kalinga III/IR64 (KIII/IR64), and Masuli/IR64 were resistant to both leaf and neck blast. However, most progenies from Masuli/MT4 showed susceptible reaction to both leaf and neck blast. Thus, rice lines form the IPB, KIII/IR64 and Masuli/IR64 will be promising resistant sources for rice blast in breeding programme.

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Acknowledgements

The authors are thankful to Department for International Developments (DFID), Plant Science Research Programme (PSRP), UK, LI-BIRD, and to the Directorate of Research, IAAS Rampur, Nepal for the financial support to this study. We are grateful to S. Gyawali for reviewing the manuscript.

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Authors and Affiliations

  1. Institute of Agriculture and Animal Science, Tribhuvan University, Chitwan, Nepal

    Krishna Datta Puri, Sunder Man Shrestha & Gopal Bahadur Khhatri Chhetri

  2. CAZS-NR, University of Wales, Bangor, UK, C/O CIMMYT-South Asia, Kathmandu, Nepal

    Krishna Dev Joshi

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  1. Krishna Datta Puri
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  2. Sunder Man Shrestha
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  3. Gopal Bahadur Khhatri Chhetri
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Correspondence to Krishna Datta Puri.

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Puri, K.D., Shrestha, S.M., Khhatri Chhetri, G.B. et al. Leaf and neck blast resistance reaction in tropical rice lines under green house condition. Euphytica 165, 523–532 (2009). https://doi.org/10.1007/s10681-008-9771-9

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