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ISSR markers detect high genetic variation among Fusarium poae isolates from Argentina and England

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Abstract

Fusarium poae is one of the Fusarium species isolated from cereal grains infected by Fusarium head blight (FHB), and in recent years it has been identified as a major FHB component. In this study, 97 F. poae isolates from Argentina (n = 62) and England (n = 35) were analysed by inter-simple sequence repeats (ISSR) to examine the genetic diversity and to determine whether intraspecific variation could be correlated with geographic and/or host origin. The molecular analysis showed high intraspecific variability within F. poae isolates, but did not reveal a clear relationship between variability and the host/geographic origin. Fusarium poae isolates from the same geographic region or host appeared in different subclusters. Conversely, isolates with the same haplotype were also collected from different geographic regions. However, we did observe subclusters consisting of isolates from Argentina only or from England only. Furthermore, a single seed sample was found to host different haplotypes. Analysis of molecular variance (AMOVA) indicated a high genetic variability in F. poae, with most of the genetic variability explained by differences within, rather than between Argentinean and English populations. This is the first report on genetic diversity of F. poae using ISSR markers. Moreover, ISSR fingerprinting generates highly polymorphic markers for F. poae and proved to be a useful and reliable assay for genetic variability studies.

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Acknowledgements

This work was supported by FONCYT-SECYT PICT-PRH 2008/110, CONICET and UNCPBA.

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Authors and Affiliations

  1. Laboratorio de Biología Funcional y Biotecnología (BIOLAB)-CEBB, Facultad de Agronomía de Azul. UNCPBA, Av. República de Italia # 780, CP 7300, Azul, Buenos Aires, Argentina

    María I. Dinolfo, Sebastian A. Stenglein & María V. Moreno

  2. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia # 1917, CP C1033AAJ, Buenos Aires, Argentina

    Sebastian A. Stenglein, María V. Moreno & Graciela L. Salerno

  3. John Innes Centre, Norwich Research Park, Colney Lane, Norwich, NR4 7UH, UK

    Paul Nicholson

  4. Central Science Laboratory, Sand Hutton, York, YO41 1LZ, UK

    Philip Jennings

  5. Fundación para Investigaciones Biológicas Aplicadas (FIBA)-CEBB, Vieytes 3103, CP 7600, Mar del Plata, Argentina

    Graciela L. Salerno

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  1. María I. Dinolfo
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Correspondence to Sebastian A. Stenglein.

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Dinolfo, M.I., Stenglein, S.A., Moreno, M.V. et al. ISSR markers detect high genetic variation among Fusarium poae isolates from Argentina and England. Eur J Plant Pathol 127, 483–491 (2010). https://doi.org/10.1007/s10658-010-9613-1

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