Abstract
Organic ultraviolet (UV) filters have often been detected in aquatic ecosystems in concentrations ranging from ng/L to μg/L. However, both their acute and chronic effects on aquatic organisms have been insufficiently explored. This study aimed to evaluate acute toxicity of some of the main UV filters used worldwide (2-ethylhexyl,4-methoxycinnamate/EHMC, avobenzone/AVO, benzophenone-3/BP-3, and octocrylene/OC), in three aquatic organisms (Artemia salina, Desmodesmus subspicatus, and Daphnia magna), and to further investigate multigenerational effects in D. magna. After acute toxicity was confirmed, daphnids were chronically exposed to environmentally relevant concentrations of UV filters for two consecutive generations (F0 and F1), and reproductive endpoints, as well as catalase (CAT) and glutathione-S-transferase (GST) activities, were assessed. EHMC showed the most toxic potential, with the lowest EC50 values for the three organisms. On the other hand, reproductive delays and a decrease in the reproduction rate were observed in the F1 generation exposed to AVO (4.4 µg/L), BP-3 (0.17 µg/L), EHMC (0.2 µg/L), and MIX. An increase of the CAT activity in organisms exposed to BP-3 and EHMC suggested induction of the antioxidant system. Although no reproductive effect was observed in the first generation, toxic effects obtained in the F1 revealed the importance of multigenerational studies and the potential harm of UV filters to the life cycle of D. magna, even at environmentally relevant concentrations. This emphasizes the need for further studies considering these levels of exposure and more realistic experimental designs to better understand their potential risks.
Environmentally relevant concentrations of Organic UV filters are not lethal to aquatic organisms, however may affect reproductive parameters in Daphnia magna though multigenerational exposures.
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Author contributions
VCSP: Conceptualization, Methodology, Writing, Data Interpretation, Original draft preparation, Investigation. MFG: Methodology, Visualization, Software, Validation. LRRM: Validation, Experimental design, Supervision. FYY: curation, Methodology, Writing- Reviewing and Editing, Supervision. AMdeF: Experimental design, Reviewing and Editing, Supervision, funding. All authors contributed to the analysis and writing of the manuscript.
Funding
This work was supported by Federal University of Technology- Paraná (UTFPR) (Paula V.’s master scholarship) and the Fundação Araucária, Brazil (UTFPR/FA-11/2016). This study also was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-Brazil – CAPES (Gomes, M. master’s scholarship). Yamamoto, FYY wish to thank the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) for her Postdoctoral Research Grant (Grant number 2016/15229-1002E).
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de Paula, V.d.C.S., Gomes, M.F., Martins, L.R.R. et al. Acute toxicity characterization of organic UV-filters and chronic exposure revealing multigenerational effects in DAPHNIA MAGNA. Ecotoxicology 31, 1413–1425 (2022). https://doi.org/10.1007/s10646-022-02598-4
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DOI: https://doi.org/10.1007/s10646-022-02598-4