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The −409 C/T Genotype of PRSS1 Protects Against Pancreatic Cancer in the Han Chinese Population

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Abstract

Background and Aims

The high mortality rate of pancreatic cancer is a bottleneck for further treatment with long-term efficacy. Thus, it is urgent to identify new methods to accurately predict the early onset of pancreatic cancer. We hypothesized that the different genotypes of cationic trypsinogen (PRSS1) gene could confer susceptibility and/or resistance to pancreatic cancer in the Han Chinese population.

Methods

The genotypes of PRSS1 were determined in 154 patients with pancreatic cancer and in a control group of 520 healthy individuals of Han Chinese descent. Clinical information was obtained, single-nucleotide polymorphisms (SNPs) of the PRSS1 gene were analyzed by direct sequencing, and the distribution of the genotypes were tested for Hardy–Weinberg equilibrium. Odds ratios and 95% confidence intervals were calculated by logistic regression analysis to estimate the associations between the different genotypes or haplotypes and the risk of pancreatic cancer.

Results

Three SNPs (−409 C/T, −204 A/C, and c.486 C/T) were identified. A case-control analysis revealed a 0.118-fold (95% CI: 0.037–0.653), 0.842-fold (95% CI: 0.177–4.010), and 0.750-fold (95% CI: 0.519–1.085) change in risk of develo** pancreatic cancer for individuals harboring these SNPs, respectively. The individuals with the −409 C/T genotype tended to have a reduced risk compared to those who carried the −409 T/T genotype. A protective effect was observed for the C−409–A−204–C486 haplotype compared to the T−409–A−204–T486 haplotype (OR = 0.115, 95% CI: 0.016–0.849) or compared to the T−409–A−204–C486 haplotype (OR = 0.090, 95% CI: 0.012–0.667). Serum levels of trypsin in patients with the −409 C/T genotype were only one-fourth that of those with the −409 T/T genotype and only one-third that of the healthy controls.

Conclusions

The −409 C/T genotype of PRSS1 was reveled to be a protective factor against pancreatic cancer in the Han Chinese population.

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Acknowledgments

Financial support from the Project Foundation of Fujian Provincial Education (JA10143), National High Technology Investigation Project Foundation of China (2008AA02Z433), National Natural Science Foundation of China (20975021, 20805006), and the Major Program Foundation of Fujian Medical University (09ZD013) is greatly acknowledged.

Conflict of interest

None.

Author information

Authors and Affiliations

  1. Department of Laboratory Medicine, The First Affiliated Hospital, Fujian Medical University, Fuzhou, 350005, China

    Qicai Liu

  2. Department of Gene Diagnosis, Fujian Medical University, Fuzhou, 350005, China

    Qicai Liu

  3. Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou, 350005, China

    **nhua Lin & Ailin Liu

  4. Department of Surgery, The First Affiliated Hospital, Fujian Medical University, Fuzhou, 350005, China

    **gfeng Liu

  5. Department of Pathology, The First Affiliated Hospital, Fujian Medical University, Fuzhou, 350005, China

    Feng Gao

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  1. Qicai Liu
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  2. **nhua Lin
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  4. Ailin Liu
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Correspondence to **nhua Lin.

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Liu, Q., Lin, X., Liu, J. et al. The −409 C/T Genotype of PRSS1 Protects Against Pancreatic Cancer in the Han Chinese Population. Dig Dis Sci 57, 573–579 (2012). https://doi.org/10.1007/s10620-011-1893-6

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  • DOI: https://doi.org/10.1007/s10620-011-1893-6

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