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Genetic monitoring of two decades of hybridization between allis shad (Alosa alosa) and twaite shad (Alosa fallax)

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Abstract

Habitat alteration has been implicated in driving hybridization between the sympatric migratory shads Alosa alosa and Alosa fallax. Morphological and molecular evidence is consistent with hybridization across the overlap** range of these species, but the temporal extent of hybrid occurrence and genetic consequences for populations have not been explored. Using eight nuclear microsatellite loci and samples collected between 1989 and 2008 in the Solway Firth (UK), we genetically identified hybrids, studied temporal changes in their frequency, and explored changes in allele frequencies of parental populations. These molecular data confirmed the hybrid status of individuals identified using morphology (number of rakers on the outer gill arch), and enabled separation of hybrids from purebred individuals. Mitochondrial cytochrome-b sequencing revealed the presence of two haplogroups, each predominantly occurring in one species. Heterospecific haplotypes were found in 22.3 and 12.8% of A. alosa and A. fallax individuals, respectively, consistent with backcrossing and suggesting that hybrids are fertile. On average, microsatellite-identified hybrids comprised 12.7% of all samples, but when individuals with cytonuclear discordance were also considered introgressed on average 25.4% of individuals were of hybrid ancestry. Overall, allelic richness remained largely unchanged within species, but there were declines in the inbreeding coefficient (F IS) of both species and episodes of significant temporal allelic frequency change. Hybrids sampled between 2004 and 2008 showed no evidence of lower fecundity relative to purebred individuals. Together, results suggest that hybridization between shad species in northern Europe is prevalent, and has been ongoing over at least two decades. The challenge is now to understand the extent to which observed patterns are linked to immigration from other populations, and the mechanisms that have prevented species collapse despite apparent hybrid fertility and longstanding introgression of neutral markers.

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Acknowledgments

We thank Jane Coghill and Keith Edwards from the Transcriptomics Facility at the University of Bristol for assistance with genoty**. This work was funded by the Esmee Fairbairn Foundation. MJG was additionally supported by Great Western Research Fellowship. We are grateful to the Galloway Fisheries Trust and the salmon netsmen of the Solway for help in obtaining samples.

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  1. Marine Biological Association of the United Kingdom, The Laboratory, Citadel Hill, Plymouth, PL1 2PB, UK

    M. T. Jolly & M. J. Genner

  2. Fish Conservation Centre, Gladshot, Haddington, EH41 4NR, Scotland, UK

    P. S. Maitland

  3. School of Biological Sciences, University of Bristol, Woodland Road, Bristol, BS8 1UG, UK

    M. J. Genner

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Correspondence to M. T. Jolly.

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Jolly, M.T., Maitland, P.S. & Genner, M.J. Genetic monitoring of two decades of hybridization between allis shad (Alosa alosa) and twaite shad (Alosa fallax). Conserv Genet 12, 1087–1100 (2011). https://doi.org/10.1007/s10592-011-0211-3

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