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Centromere targeting of alien CENH3s in Arabidopsis and tobacco cells

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Abstract

The centromere is a region utilized for spindle attachment on a eukaryotic chromosome and essential for accurate chromatid segregation. In most eukaryotes, centromeres have specific DNA sequences and are capable of assembling specific proteins to form a complex called the kinetochore. Among these proteins, centromeric histone H3 (CENH3) is one of the most fundamental, since CENH3s have been found in all investigated functional centromeres and recruits other centromeric proteins. In this study, the localization of alien CENH3s were analyzed in Arabidopsis and tobacco-cultured cells to determine the interaction between species-specific centromeric DNA and CENH3. Results showed that CENH3 of Arabidopsis and tobacco were localized on centromeres in the tobacco-cultured cells, unlike the case with CENH3 of rice and Luzula. In addition to these CENH3s, CENH3 of Luzula was partially localized in the Arabidopsis cultured cells. These data suggest that only evolutionally close CENH3s are able to target centromeres in alien species. Furthermore, the ability to target alien centromeres of histone fold domains was investigated using amino-terminal deleted CENH3s.

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Abbreviations

2, 4-D:

2, 4-dichlorophenoxyacetic acid

Cen:

centromeric

CENH3:

centromeric histone H3

Disp:

dispersed

GFP:

green fluorescent protein

HFD:

histone fold domains

HTR12:

centromeric histone H3 of Arabidopsis thaliana

LnCENH3:

centromeric histone H3 of Luzula nivea

LS medium:

Linsmaier and Skoog’s medium

MS medium:

Murashige and Skoog’s medium

NtCENH3:

centromeric histone H3 of Nicotiana tabaccum

OsCENH3:

centromeric histone H3 of Oryza sativa

T-DNA:

transfer DNA

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Acknowledgments

This work was supported by a grant from the Wesco Scientific Promotion Foundation.

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Authors and Affiliations

  1. Research Institute for Bioresources, Okayama University, Kurashiki, 710-0046, Japan

    Kiyotaka Nagaki, Kaori Terada, Munenori Wakimoto, Kazunari Kashihara & Minoru Murata

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  1. Kiyotaka Nagaki
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  2. Kaori Terada
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  3. Munenori Wakimoto
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  4. Kazunari Kashihara
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  5. Minoru Murata
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Correspondence to Kiyotaka Nagaki.

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Responsible Editor: Hans de Jong.

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Nagaki, K., Terada, K., Wakimoto, M. et al. Centromere targeting of alien CENH3s in Arabidopsis and tobacco cells. Chromosome Res 18, 203–211 (2010). https://doi.org/10.1007/s10577-009-9108-0

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  • DOI: https://doi.org/10.1007/s10577-009-9108-0

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