Abstract
Owing to their inherent biocompatibility and biodegradability, biopolymer-based materials are a good replacement for synthetic materials. We prepared cellulose-based films blended with various biopolymers by co-dissolution in 1-ethyl-3-methylimidazolium acetate followed by regeneration in ethanol. Cellulose was blended with bacterial cellulose, β-cyclodextrin, dextran, starch, agarose, agar, arabic gum, κ-carrageenan, chitosan, tragacanth gum, xanthan gum, xylan, lignin, gelatin, collagen, and silk. Physicochemical properties including surface morphology, thermal properties, optical properties, and hydrophilicity/hydrophobicity of the blended films were highly dependent on the biopolymer types. The altered properties of the blended films influenced the adsorption capacity for dyes and proteins and cytotoxicity. The adsorption capacity of a cellulose/chitosan film for methyl orange was 9.1-fold higher, that of a cellulose/silk film for crystal violet was 7.9-fold higher, and that of a cellulose/chitosan film for lysozyme was 7.9-fold higher than that of cellulose film. Gelatin as a blending biopolymer most efficiently enhanced cell viability.
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References
Acharya C, Ghosh SK, Kundu SC (2008) Silk fibroin protein from mulberry and non-mulberry silkworms: cytotoxicity, biocompatibility and kinetics of L929 murine fibroblast adhesion. J Mater Sci Mater Med 19(8):2827–2836. https://doi.org/10.1007/s10856-008-3408-3
Awad HA, Wickham MQ, Leddy HA, Gimble JM, Guilak F (2004) Chondrogenic differentiation of adipose-derived adult stem cells in agarose, alginate, and gelatin scaffolds. Biomaterials 25(16):3211–3222. https://doi.org/10.1016/j.biomaterials.2003.10.045
Bajpai AK, Shukla SK, Bhanu S, Kankane S (2008) Responsive polymers in controlled drug delivery. Prog Polym Sci 33(11):1088–1118. https://doi.org/10.1016/j.progpolymsci.2008.07.005
Bangyekan C, Aht-Ong D, Srikulkit K (2006) Preparation and properties evaluation of chitosan-coated cassava starch films. Carbohydr Polym 63(1):61–71. https://doi.org/10.1016/j.carbpol.2005.07.032
Chinabhark K, Benjakul S, Prodpran T (2007) Effect of pH on the properties of protein-based film from bigeye snapper (Priacanthus tayenus) surimi. Bioresour Technol 98(1):221–225. https://doi.org/10.1016/j.biortech.2005.11.012
DeFrates K, Markiewicz T, Callaway K, Xue Y, Stanton J, Salas-de la Cruz D, Hu X (2017) Structure–property relationships of Thai silk–microcrystalline cellulose biocomposite materials fabricated from ionic liquid. Int J Biol Macromol 104:919–928. https://doi.org/10.1016/j.ijbiomac.2017.06.103
Ebringerová A, Heinze T (2000) Xylan and xylan derivatives—biopolymers with valuable properties, 1. Naturally occurring xylans structures, isolation procedures and properties. Macromol Rapid Commun 21(9):542–556. https://doi.org/10.1002/1521-3927(20000601)21:9%3c542:AID-MARC542%3e3.0.CO;2-7
Eraković S, Veljović D, Diouf PN, Stevanović T, Mitrić M, Janaćković D, Matić IZ, Juraniće ZD, Mišković-Stanković V (2012) The effect of lignin on the structure and characteristics of composite coatings electrodeposited on titanium. Prog Org Coat 75(4):275–283. https://doi.org/10.1016/j.porgcoat.2012.07.005
Farris S, Introzzi L, Biagioni P, Holz T, Schiraldi A, Piergiovanni L (2011) Wetting of biopolymer coatings: contact angle kinetics and image analysis investigation. Langmuir 27(12):7563–7574. https://doi.org/10.1021/la2017006
Fernandes SCM, Oliveira L, Freire CSR, Silvestre AJD, Neto CP, Gandini A, Desbriéres J (2009) Novel transparent nanocomposite films based on chitosan and bacterial cellulose. Green Chem 11(12):2023–2029. https://doi.org/10.1039/B919112G
Foo CWP, Bini E, Hensman J, Knight DP, Lewis RV, Kaplan DL (2006) Role of pH and charge on silk protein assembly in insects and spiders. Appl Phys A Mater Sci Process 82(2):223–233. https://doi.org/10.1007/s00339-005-3426-7
Freemantle M (1998) Designer solvents–ionic liquids may boost clean technology development. Chem Eng News 76(13):32–37. https://doi.org/10.1021/cen-v076n013.p032
Fu W, Liu Z, Feng B, Hu R, He X, Wang H, Yin M, Huang H, Zhang H, Wang W (2014) Electrospun gelatin/PCL and collagen/PLCL scaffolds for vascular tissue engineering. Int J Nanomed 9:2335–2344. https://doi.org/10.2147/IJN.S61375
Haafiz MKM, Hassan A, Zakaria Z, Inuwaa IM (2014) Isolation and characterization of cellulose nanowhiskers from oil palm biomass microcrystalline cellulose. Carbohydr Polym 103(15):119–125. https://doi.org/10.1016/j.carbpol.2013.11.055
Hamcerencu M, Desbrieres J, Popa M, Khoukh A, Riess G (2007) New unsaturated derivatives of Xanthan gum: synthesis and characterization. Polymer 48(7):1921–1929. https://doi.org/10.1016/j.polymer.2007.01.048
Harmita H, Karthikeyan KG, Pan X (2009) Copper and cadmium sorption onto kraft and organosolv lignins. Bioresour Technol 100(24):6183–6191. https://doi.org/10.1016/j.biortech.2009.06.093
Hu X, Kaplan D, Cebe P (2006) Determining beta-sheet crystallinity in fibrous proteins by thermal analysis and infrared spectroscopy. Macromolecules 39(18):6161–6170. https://doi.org/10.1021/ma0610109
Hunt NC, Grover LM (2010) Cell encapsulation using biopolymer gels for regenerative medicine. Biotechnol Lett 32(6):733–742. https://doi.org/10.1007/s10529-010-0221-0
Imamura K, Shimomura M, Nagai S, Akamatsu M, Nakanishi K (2008) Adsorption characteristics of various proteins to a titanium surface. J Biosci Bioeng 106(3):273–278. https://doi.org/10.1263/jbb.106.273
Kim J, Somorjai GA (2003) Molecular packing of lysozyme, fibrinogen, and bovine serum albumin on hydrophilic and hydrophobic surfaces studied by infrared-visible sum frequency generation and fluorescence microscopy. J Am Chem Soc 125(10):3150–3158. https://doi.org/10.1021/ja028987n
Kim MH, An S, Won K, Kim HJ, Lee SH (2012) Entrapment of enzymes into cellulose–biopolymer composite hydrogel beads using biocompatible ionic liquid. J Mol Catal B Enzym 75:68–72. https://doi.org/10.1016/j.molcatb.2011.11.011
Kim Y, Park S, Kim MH, Choi Y, Yang Y, Kim HJ, Kim H, Kim H, Song K, Lee SH (2013) Ultrasound-assisted extraction of lipids from Chlorella vulgaris using [Bmim][MeSO4]. Biomass Bioenergy 56:99–103. https://doi.org/10.1016/j.biombioe.2013.04.022
Kim SH, Kim MH, Kim JH, Park S, Kim H, Won K, Lee SH (2015) Preparation of artificial wood films with controlled biodegradability. J Appl Polym Sci 132(25):42109. https://doi.org/10.1002/app.42109
Kim JH, Park S, Kim H, Kim HJ, Yang Y, Kim YH, Jung S, Kan E, Lee SH (2017) Alginate/bacterial cellulose nanocomposite beads prepared using Gluconacetobacter xylinus and their application in lipase immobilization. Carbohydr Polym 157(10):137–145. https://doi.org/10.1016/j.carbpol.2016.09.074
Lai C, Chen Y, Zhang S (2013) Study on chitosan-lactate sponges with oriented pores as potential wound dressing. Mater Sci Appl 4(8):458–470. https://doi.org/10.4236/msa.2013.48056
Lammel AS, Hu X, Park S, Kaplan DL, Scheibel TR (2010) Controlling silk fibroin particle features for drug delivery. Biomaterials 31(16):4583–4591. https://doi.org/10.1016/j.biomaterials.2010.02.024
Lee SH, Doherty TV, Linhardt RJ, Dordick JS (2009) Ionic liquid-mediated selective extraction of lignin from wood leading to enhanced enzymatic cellulose hydrolysis. Biotechnol Bioeng 102(5):1368–1376. https://doi.org/10.1002/bit.22179
Lee SC, Tran TMT, Choi JW, Won K (2019) Lignin for white natural sunscreens. Int J Biol Macromol 122(1):549–554. https://doi.org/10.1016/j.ijbiomac.2018.10.184
Lepifre S, Froment M, Cazaux F, Houot S, Lourdin D, Coqueret IX, Lapierre C, Baumberger S (2004) Lignin incorporation combined with electron-beam irradiation improves the surface water resistance of starch films. Biomacromol 5(5):1678–1686. https://doi.org/10.1021/bm040005e
Li J, Nie S (2016) The functional and nutritional aspects of hydrocolloids in foods. Food Hydrocolloids 53:46–61. https://doi.org/10.1016/j.foodhyd.2015.01.035
Li L, **ong Y, Yu S, Liu S, Liu F, **e C (2015) Facile preparation for robust and freestanding silk fibroin films in a 1-butyl-3-methyl imidazolium acetate ionic liquid system. J Appl Polym Sci 132(47):42822. https://doi.org/10.1002/app.42822
Liu C, Bai R (2005) Preparation of chitosan/cellulose acetate blend hollow fibers for adsorptive performance. J Membr Sci 267(1–2):68–77. https://doi.org/10.1016/j.memsci.2005.06.001
Liu Z, Huang H (2016) Preparation and characterization of cellulose composite hydrogels from tea residue and carbohydrate additives. Carbohydr Polym 147(20):226–233. https://doi.org/10.1016/j.carbpol.2016.03.100
Manawi Y, Kochkodan V, Mohammad AW, Atieha MA (2017) Arabic gum as a novel pore-forming and hydrophilic agent in polysulfone membranes. J Membr Sci 529(1):95–104. https://doi.org/10.1016/j.memsci.2017.02.002
Mendes LC, Ribeiro GL, Marques RC (2012) In situ hydroxyapatite synthesis: influence of collagen on its structural and morphological characteristic. Mater Sci Appl 3(8):580–586. https://doi.org/10.4236/msa.2012.38083
Muginova SV, Myasnikov DA, Kazarianc SG, Shekhovtsova TN (2016) Evaluation of novel applications of cellulose hydrogel films reconstituted from acetate and chloride of 1-butyl-3-methylimidazolium by comparing their optical, mechanical, and adsorption properties. Mater Today Commun 8:108–117. https://doi.org/10.1016/j.mtcomm.2016.06.005
Muhamad II, Fen LS, Hui NH, Mustapha NA (2011) Genipin-cross-linked kappa-carrageenan/carboxymethyl cellulose beads and effects on beta-carotene release. Carbohydr Polym 83(3):1207–1212. https://doi.org/10.1016/j.carbpol.2010.09.021
Nair V, Panigrahy A, Vinu R (2014) Development of novel chitosan–lignin composites for adsorption of dyes and metal ions from wastewater. Chem Eng J 254(15):491–502. https://doi.org/10.1016/j.cej.2014.05.045
Numata K, Katashima T, Sakai T (2011) State of water, molecular structure, and cytotoxicity of silk hydrogels. Biomacromol 12(6):2137–2144. https://doi.org/10.1021/bm200221u
Pang J, Liu X, Zhang X, Wu Y, Sun R (2013) Fabrication of cellulose film with enhanced mechanical properties in ionic liquid 1-allyl-3-methylimidaxolium chloride (AmimCl). Materials 6(4):1270–1284. https://doi.org/10.3390/ma6041270
Pang J, Liu X, Wu M, Wu Y, Zhang X, Sun R (2014) Fabrication and characterization of regenerated cellulose films using different ionic liquids. J Spectrosc 2014:214057. https://doi.org/10.1155/2014/214057
Park S, Kim SH, Kim JH, Yu H, Kim HJ, Yang Y, Kim H, Kim YH, Ha SH, Lee SH (2015a) Application of cellulose/lignin hydrogel beads as novel supports for immobilizing lipase. J Mol Catal B Enzym 119:33–39. https://doi.org/10.1016/j.molcatb.2015.05.014
Park S, Kim SH, Won K, Choi JW, Kim YH, Kim HJ, Yang Y, Lee SH (2015b) Wood mimetic hydrogel beads for enzyme immobilization. Carbohydr Polym 115(22):223–229. https://doi.org/10.1016/j.carbpol.2014.08.096
Park S, Lih E, Park K, Joung YK, Han DK (2017) Biopolymer-based functional composites for medical applications. Prog Polym Sci 68:77–105. https://doi.org/10.1016/j.progpolymsci.2016.12.003
Park S, Doan YYN, Koo Y, Oh KK, Lee SH (2018) Ionic liquids as cosolvents for the lipase-catalyzed kinetic resolution of ketoprofen. Mol Catal 459:113–118. https://doi.org/10.1016/j.mcat.2018.09.001
Park S, Oh KK, Lee SH (2019) Biopolymer-based composite materials prepared using ionic liquids. Adv Biochem Eng Biotechnol 168:133–176. https://doi.org/10.1007/10_2018_78
Pawlak A, Mucha M (2003) Thermogravimetric and FTIR studies of chitosan blends. Thermochim Acta 396(1–2):153–166. https://doi.org/10.1016/S0040-6031(02)00523-3
Richards D, Ivanisevic A (2012) Inorganic material coatings and their effect on cytotoxicity. Chem Soc Rev 41(6):2052–2060. https://doi.org/10.1039/C1CS15252A
Roach P, Farrar D, Perry CC (2005) Interpretation of protein adsorption: surface-induced conformational changes. J Am Chem Soc 127(22):8168–8173. https://doi.org/10.1021/ja042898o
Sheldon RA, Lau RM, Sorgedrager MJ, van Rantwijk F, Seddon KR (2002) Biocatalysis in ionic liquids. Green Chem 4(2):147–151. https://doi.org/10.1039/B110008B
Silva SS, Popa EG, Gomes ME, Oliveira MB, Nayak S, Subia B, Mano JF, Kundu SC, Reis RL (2013) Silk hydrogels from non-mulberry and mulberry silkworm cocoons processed with ionic liquids. Acta Biomater 9(11):8972–8982. https://doi.org/10.1016/j.actbio.2013.06.044
Simmons TJ, Lee SH, Miao J, Miyauchi M, Park T, Bale SS, Pangule R, Bult J, Martin JG, Dordick JS, Linhardt RJ (2011) Preparation of synthetic wood composites using ionic liquids. Wood Sci Technol 45(4):719–733. https://doi.org/10.1007/s00226-010-0395-6
Singh B, Sharma V (2014) Influence of polymer network parameters of tragacanth gum-based pH responsive hydrogels on drug delivery. Carbohydr Polym 101(30):928–940. https://doi.org/10.1016/j.carbpol.2013.10.022
Sionkowska A, Planecka A (2011) The influence of UV radiation on silk fibroin. Polym Degrad Stabil 96(4):523–528. https://doi.org/10.1016/j.polymdegradstab.2011.01.001
Stefanescu C, Daly WH, Negulescu II (2012) Biocomposite films prepared from ionic liquid solutions of chitosan and cellulose. Carbohydr Polym 87(1):435–443. https://doi.org/10.1016/j.carbpol.2011.08.003
Tan XY, Hamid SBA, Lai CW (2015) Preparation of high crystallinity cellulose nanocrystals (CNCs) by ionic liquid solvolysis. Biomass Bioenerg 81:584–591. https://doi.org/10.1016/j.biombioe.2015.08.016
Ugartondo V, Mitjans M, Vinardell MP (2008) Comparative antioxidant and cytotoxic effects of lignins from different sources. Bioresour Technol 99(14):6683–6687. https://doi.org/10.1016/j.biortech.2007.11.038
Ventura SPM, de Barros RLF, de Pinho Barbosa JM, Soares CMF, Lima ÁS, Coutinho JAP (2012) Production and purification of an extracellular lipolytic enzyme using ionic liquid-based aqueous two-phase systems. Green Chem 14(3):734–740. https://doi.org/10.1039/C2GC16428K
Wendler F, Persin Z, Stana-Kleinschek K, Reischl M, Ribitsch V, Bohn A, Fink H, Meister F (2011) Morphology of polysaccharide blend fibers shaped from NaOH, N-methylmorpholine-N-oxide and 1-ethyl-3-methylimidazolium acetate. Cellulose 18(5):1165–1178. https://doi.org/10.1007/s10570-011-9559-2
Williams SJ, Wang Q, MacGregor RR, Siahaan TJ, Stehno-Bittel L, Berkland C (2009) Adhesion of pancreatic beta cells to biopolymer films. Biopolymers 91(8):676–685. https://doi.org/10.1002/bip.21196
Wu T, Farnood R, O’Kelly K, Chena B (2014) Mechanical behavior of transparent nanofibrillar cellulose–chitosan nanocomposite films in dry and wet conditions. J Mech Behav Biomed Mater 32:279–286. https://doi.org/10.1016/j.jmbbm.2014.01.014
Xu X, Luo L, Liu C, McClements DJ (2017) Utilization of anionic polysaccharides to improve the stability of rice glutelin emulsions: impact of polysaccharide type, pH, salt, and temperature. Food Hydrocoll 64:112–122. https://doi.org/10.1016/j.foodhyd.2016.11.005
Yamane C, Aoyagi T, Ago M, Sato K, Okajima K, Takahashi T (2006) Two different surface properties of regenerated cellulose due to structural anisotropy. Polym J 38(8):819–826. https://doi.org/10.1295/polymj.pj2005187
Yu L, Dean K, Li L (2006) Polymer blends and composites from renewable resources. Prog Polym Sci 31(6):576–602. https://doi.org/10.1016/j.progpolymsci.2006.03.002
Zhang M, Ding C, Chen L, Huang L (2014) The preparation of cellulose/collagen composite films using 1-ethyl-3-methylimidazolium acetate as a solvent. BioResources 9(1):756–771. https://doi.org/10.15376/biores.9.1.756-771
Acknowledgments
This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education [grant number: 2018R1D1A1B07050163], and by the Technology Development Program to Solve Climate Changes of the NRF, funded by the Ministry of Science and ICT [grant numbers: 2017M1A2A2087627 and 2017M1A2A2087647]. This work was also supported by research grant from the Ministry of Trade, Industry and Energy through the Korean Evaluation Institute of Industrial Technology [grant number: 20002810].
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Park, S., Oh, Y., Yun, J. et al. Characterization of blended cellulose/biopolymer films prepared using ionic liquid. Cellulose 27, 5101–5119 (2020). https://doi.org/10.1007/s10570-020-03152-3
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DOI: https://doi.org/10.1007/s10570-020-03152-3