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A rice mutant defective in antioxidant-defense system and sodium homeostasis possesses increased sensitivity to salt stress

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Biologia Plantarum

Abstract

Screening salt-sensitive mutants is a powerful method to identify genes associated with salt tolerance. We used forward genetic screening with sodium azide-mutated rice (Oryza sativa L. cv. Tainung 67) to identify mutants showing hypersensitivity to salt stress. A new mutant line, named salt hypersensitive 1 (shs1) and exhibiting a severe salt-sensitivity when grown under a high NaCl concentration, was identified; the salt hypersensitivity was caused by duplicate recessive epistasis with mutations likely in two different loci. The shs1 salt sensitive phenotypes included a decreased seed germination rate, reduced shoot height and root length, severe and quick wilting, and overaccumulation of sodium ions in shoots as compared with wild-type plants. In addition, shs1 showed a decreased photosynthetic efficiency and enhanced hydrogen peroxide (H2O2) production under the salt stress. An increased superoxide dismutase activity and decreased catalase activity were responsible for the hyperaccumulation of H2O2 in shs1. The hypersensitivity of shs1 to the salt stress might be caused by an impaired antioxidant machinery and cellular Na+ homeostasis.

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Abbreviations

AsA:

ascorbate

APX:

ascorbate peroxidase

CAT:

catalase

DAB:

3,3-diaminobenzidine

Fv/Fm :

variable to maximum chlorophyll a fluorescence ratio

GR:

glutathione reductase

H2O2 :

hydrogen peroxide

NBT:

nitroblue tetrazolium

NB:

Nona Bokra

OH· :

hydroxyl radical

ROS:

reactive oxygen species

SOD:

superoxide dismutase

shs1 :

salt hypersensitive 1

O2 ·− :

superoxide

TNG67:

Tainung 67

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Authors and Affiliations

  1. Department of Agricultural Chemistry, National Taiwan University, Taipei, 10617, Taiwan

    K. -C. Lin, N. N. P. Chandrika, T. -M. Wu & C. -Y. Hong

  2. Taiwan Agricultural Research Institute, Wufeng, Taichung, 41362, Taiwan

    W. -S. Jwo & M. -H. Lai

  3. Department of Agronomy, National Chung-Hsing University, Taichung, 40227, Taiwan

    C. -S. Wang

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  1. K. -C. Lin
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  2. W. -S. Jwo
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  3. N. N. P. Chandrika
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  4. T. -M. Wu
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  5. M. -H. Lai
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  6. C. -S. Wang
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  7. C. -Y. Hong
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Correspondence to C. -Y. Hong.

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Acknowledgments: This work was partly supported by a research grant NSC 101-2313-B-002-008-MY3 from the Ministry of Science and Technology of the Republic of China to C.Y. Hong. N.N.P. Chandrika was supported by the National Taiwan University Postdoctoral Research Fellowships. The first two authors contributed equally to this paper.

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Lin, K.C., Jwo, W.S., Chandrika, N.N.P. et al. A rice mutant defective in antioxidant-defense system and sodium homeostasis possesses increased sensitivity to salt stress. Biol Plant 60, 86–94 (2016). https://doi.org/10.1007/s10535-015-0561-7

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  • DOI: https://doi.org/10.1007/s10535-015-0561-7

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