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Development of thirty new polymorphic microsatellite primers for Paeonia suffruticosa

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Biologia Plantarum

Abstract

Four new microsatellite primer pairs were developed in tree peony (Paeonia suffruticosa) based on the database mining and other twenty-six primer pairs by fast isolation by AFLP of sequences containing repeats (FIASCO) method. The polymorphism of each locus was further evaluated in 40 individuals of one population plus 5 tree peony related species. The number of alleles per locus ranged from 3 to 7 and the expected (He) and observed (Ho) heterozygosity at each locus ranged from 0.42 to 0.78 and 0.28 to 0.59, respectively. These microsatellite markers will be useful for investigating genetic diversity and studies of population genetic structure of tree peony.

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Abbreviations

AFLP:

amplified fragment length polymorphism

CTAB:

cetyltrimethylammonium bromide

FIASCO:

fast isolation by AFLP of sequences containing repeats

ISSR:

inter-simple sequence repeat

RAPD:

random amplified polymorphic DNA

SRAP:

sequence-related amplified polymorphism

SSR:

microsatellite or simple sequence repeat

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Acknowledgements

This work was supported by Natural Science Foundation of China (NSFC: 30800742 and 31070620), Program for Science and Technology Innovation Talents in Universities of Henan Province (2010HASTIT002) and Young Teacher Funding Program of Henan Higher school (2010GGJS-072).

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Authors and Affiliations

  1. College of Agriculture, Henan University of Science and Technology, 70 Tian** Road, Jianxi District, Luoyang, 471003, Henan Province, P.R. China

    X. -G. Hou & S. -P. Cheng

  2. College of Forestry, Henan University of Science and Technology, 70 Tian** Road, Jianxi District, Luoyang, 471003, Henan Province, P.R. China

    D. -L. Guo & J. -Y. Zhang

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  1. X. -G. Hou
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  2. D. -L. Guo
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  3. S. -P. Cheng
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  4. J. -Y. Zhang
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Correspondence to D. -L. Guo.

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Hou, X.G., Guo, D.L., Cheng, S.P. et al. Development of thirty new polymorphic microsatellite primers for Paeonia suffruticosa . Biol Plant 55, 708–710 (2011). https://doi.org/10.1007/s10535-011-0172-x

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  • DOI: https://doi.org/10.1007/s10535-011-0172-x

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