Abstract
To evaluate the genetic diversity of some Vicia species, seed proteins of 160 accessions (30 of Vicia faba, 15 of V. narbonensis, 82 of V. sativa and 25 of V. ervilia and 8 accessions of other Vicia species) were analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The dendrogram showed that the two outcrossing species V. faba and V. villosa were the most distant among all species (average percent disagreement value PDV 0.47 and 0.45, respectively). The tree was divided into small clusters of two species each. V. narbonensis fell in one cluster with V. michausai (at PDV = 0.35) and V. lutea (var. hirta) fell in one cluster with V. serococorpes (at PDV = 0.32) whereas, V. ervilia fell in one cluster with V. sativa (at PDV = 0.27). The V. sativa subspecies, however, were closely related (PDV < 0.1). In general, this study did not prove any relationship between the studied storage proteins and the geographical distribution or ecological needs of the studied accessions.
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Abbreviations
- PVD:
-
percent disagreement value
- SDS-PAGE:
-
sodium dodecyl sulphate polyacrylamide gel electrophoresis
- UPGMA:
-
unweighted pair group method with arithmetic averages
References
Ahmad, M., Fautrier, A.G., Burritt, D.J., McNeil, D.L.: Genetic diversity and relationships in Lens species and their F1 inter-specific hybrids as determined by SDS-PAGE.-New Zeal. J. Crop hort. Sci. 25: 99–108, 1997.
Bertozo, M.R., Valls, J.F.M.: Seed storage protein electrophoresis in Arachis pintoi and A. repens (Leguminosae) for evaluating genetic diversity.-Genet. Resour. Crop Evol. 48: 121–130, 2001.
Crawford, D.J.: Plant Molecular Systematics, Macromolecular Approaches.-John Wiley & Sons, New York 1990.
Gardiner, S.E., Forde, M.B.: Identification of cultivars of grasses and forage legumes by SDS-PAGE of seed proteins.-In: Linsken, H.F., Jackson, J.F. (ed.): Seed Analysis. Modern Methods of Plant Analysis. Pp. 43–61. Springer-Verlag, Berlin 1992.
Haider, A.S., El-Shanshoury, A.R.: Variability of storage proteins and esterase isozymes in Vicia sativa subspecies.-Biol. Plant. 43: 205–209, 2000.
Jaaska, V.: Isoenzyme diversity and phylogenetic affinities in Vicia subgenus Vicia (Fabaceae).-Genet. Resour. Crop Evol. 44: 557–574, 1997.
Ladizinsky, G., Hymowitz, T.: Seed protein electrophoresis in taxonomic and evolutionary studies.-Theor. appl. Genet. 51: 145–151, 1979.
Ladizinsky, G., Waines, G.: Seed protein polymorphism in Vicia sativa aggregate (Fabaceae).-Plant Syst. Evol. 41: 1–4, 1982.
Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4.-Nature 227: 680–685, 1970.
Maquet, A., Zoro, I., Rocha, O., Baudoin, J.: Case studies on breeding systems and its consequences for germplasm conservation: I. Isozyme diversity in wild Lima beans populations in Central Costa Rica.-Genet. Resour. Crop Evol. 43: 309–318, 1996.
MirAli, N.: Protein Improvement in Triticum turgidum (Desf)-PhD Thesis, University of Newcastle, Newcastle 1987.
MirAli, N.: Detection of heterogeneity within old and modern durum and bread wheat grown in Syria using the A-PAGE and SDS-PAGE electrophoretic techniques.-Plant Varieties Seeds 13: 149–157, 2000.
Pearce, S.R., Harrison, G., Li, D., Heslop-Harrison, J.S., Kumar, A., Flavell, A.J.: The Ty1-copia group retrotransposons in Vicia species: copy number, sequence heterogeneity and chromosomal location.-Mol. gen. Genet. 250: 305–315, 1996.
Polignano, G.B., Splendido, R., Perrino, P.: Seed storage protein diversity in faba bean (Vicia faba L.) entries from Ethiopia and Afghanistan.-J. Genet. Breed. 44: 31–38, 1990.
Potokina, E., Endo, Y., Eggi, E., Ohashi, H.: Electrophoretic patterns of seed proteins in the East Asian Vicia species (Leguminosae) and their systematic utility.-J. jap. Bot. 78: 29–37, 2003.
Potokina, E., Tomooka, N., Vaughan, D.A., Alexandrova, T., Xu, R.Q.: Phylogeny of Vicia subgenus Vicia (Fabaceae) based on analysis of RAPDs and RFLP of PCR-amplified chloroplast genes.-Genet. Resour. Crop Evol. 46: 149–161, 1999.
Potokina, E., Vaughan, D.A., Eggi, E., Tomooka, N.: Population diversity of the Vicia sativa (Fabaceae) in the flora of the former USSR deduced from RAPD and seed protein analyses.-Genet. Resour. Crop Evol. 47: 171–183, 2000.
Rogal, S., Javornik, B.: Seed protein variation for identification of common buckwheat (Fagopyrum esculentum Moench) cultivars.-Euphytica 87: 111–117, 1996.
Vargas, E.M., Macaya, G., Baudoin, J.P., Rocha, O.J.: Case studies on breeding systems and its consequences for germplasm conservation: 3. Electrophoretic mobility of phaseolins in wild populations of Lima beans (Phaseolus lunatus L.) in the Central Valley of Costa Rica.-Genet. Resour. Crop Evol. 48: 109–120, 2001.
Young, N.D., Mudge, J., Ellis, T.H.N.: Legume genomes: more than peas in a pod.-Curr. Opin. Plant Biol. 6: 199–204, 2003.
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Mirali, N., El-Khouri, S. & Rizq, F. Genetic diversity and relationships in some Vicia species as determined by SDS-PAGE of seed proteins. Biol Plant 51, 660–666 (2007). https://doi.org/10.1007/s10535-007-0139-0
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DOI: https://doi.org/10.1007/s10535-007-0139-0