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Production of saponins from Panax ginseng suspension and adventitious root cultures

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Biologia Plantarum

Abstract

Biomass growth and ginsenoside production in cell suspension and adventitious roots of Panax ginseng C.A. Meyer cultures cultivated both in Erlenmayer flasks and a 3 dm3 bioreactor were studied. The maximum content of ginsenosides was found in the suspension culture cultivated in the bioreactor (4.34 % dry mass), however the saponin content was limited to two major ginsenosides, Rb1 and Rg1. The production of ginsenosides in adventitious roots was lower (1.45 or 1.72 % dry mass), nevertheless, the full range of ginsenosides was detected.

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Abbreviations

IBA:

indole-3-butric acid

NAA:

naphthalene acetic acid

2,4-D:

2,4-dichlorphenoxyacetic acid

SH medium:

Schenk and Hildebrandt medium

MS medium:

Murashige and Skoog medium

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Correspondence to T. Vanek.

Additional information

This work was supported by 521/02/P064, COST 843.10, ME671 and Z4 055 905 projects.

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Langhansova, L., Marsik, P. & Vanek, T. Production of saponins from Panax ginseng suspension and adventitious root cultures. Biol Plant 49, 463–465 (2005). https://doi.org/10.1007/s10535-005-0030-9

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