SpringerLink
Log in

Sublethal concentration of H2O2 enhances the protective effect of mesenchymal stem cells in rat model of spinal cord injury

  • Original Research Paper
  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Objective

To investigate the effect of H2O2 on the migration and antioxidant defense of mesenchymal stem cells (MSCs) and the neurotrophic effects of H2O2-treated MSCs on spinal cord injury (SCI).

Results

Sublethal concentrations of H2O2 decreased cell migration and expression of CXCR4 and CCR2 as well as Nrf2 expression in MSCs. In the second phase, transplantation of treated and untreated MSCs to SCI caused minor changes in locomotor dysfunction. There was a significantly difference between cell-treated and spinal cord injury groups in expression of BDNF (brain-derived neurotrophic factor). Transplantation of H2O2-treated cells caused an increase in BDNF expression compared to non-treated cells.

Conclusion

Transplantation of H2O2-treated stem cells may have protective effects against SCI through by increasing neurotrophic factors.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price includes VAT (Germany)

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  • Basso DM, Beattie MS, Bresnahan JC (1995) A sensitive and reliable locomotor rating scale for open field testing in rats. J Neurotrauma 12:1–21

    Article  CAS  PubMed  Google Scholar 

  • Chang C-P, Chio C-C, Cheong C-U, Chao C-M, Cheng B-C, Lin M-T (2013) Hypoxic preconditioning enhances the therapeutic potential of the secretome from cultured human mesenchymal stem cells in experimental traumatic brain injury. Clin Sci 124:165–176

    Article  CAS  PubMed  Google Scholar 

  • Der Sarkissian S, Lévesque T, Noiseux N (2017) Optimizing stem cells for cardiac repair: current status and new frontiers in regenerative cardiology. World J Stem Cells 9:9

    Article  Google Scholar 

  • Devine SM, Cobbs C, Jennings M, Bartholomew A, Hoffman R (2003) Mesenchymal stem cells distribute to a wide range of tissues following systemic infusion into nonhuman primates. Blood 101:2999–3001

    Article  CAS  PubMed  Google Scholar 

  • Himes BT et al (2006) Recovery of function following grafting of human bone marrow-derived stromal cells into the injured spinal cord. Neurorehab Neural Repair 20:278–296

    Article  Google Scholar 

  • Imura T, Tomiyasu M, Otsuru N, Nakagawa K, Otsuka T (2017) Hypoxic preconditioning increases the neuroprotective effects of mesenchymal stem cells in a rat model of spinal cord injury. J Stem Cell Res Ther 7:2

    Article  Google Scholar 

  • Jang Y-Y, Sharkis SJ (2007) A low level of reactive oxygen species selects for primitive hematopoietic stem cells that may reside in the low-oxygenic niche. Blood 110:3056–3063

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Le Belle JE et al (2011) Proliferative neural stem cells have high endogenous ROS levels that regulate self-renewal and neurogenesis in a PI3K/Akt-dependent manner. Cell Stem Cell 8:59–71

    Article  PubMed  PubMed Central  Google Scholar 

  • Lin T-M, Tsai J-L, Lin S-D, Lai C-S, Chang C-C (2005) Accelerated growth and prolonged lifespan of adipose tissue-derived human mesenchymal stem cells in a medium using reduced calcium and antioxidants. Stem Cells Dev 14:92–102

    Article  CAS  PubMed  Google Scholar 

  • Nardi NB, da Silva LM (2008) Mesenchymal stem cells: isolation, in vitro expansion and characterization. Stem cells. Springer, Berlin, pp 249–282

    Chapter  Google Scholar 

  • Nishimoto T, Matsumoto A, Kihara T, Akaike A, Sugimoto H (2009) Protective effect of H2O2 against subsequent H2O2-induced cytotoxicity involves activation of the PI3K-Akt signaling pathway. Cell Mol Biol 56:OL1447

    Google Scholar 

  • Pourjafar M, Saidijam M, Mansouri K, Malih S, Nejad RT, Shabab N, Najafi R (2016) Cytoprotective effects of endothelin-1 on mesenchymal stem cells: an in vitro study. Clin Exp Pharmacol Physiol l43:769–776

    Article  Google Scholar 

  • Zhang J et al (2012) Hydrogen peroxide preconditioning enhances the therapeutic efficacy of Wharton’s Jelly mesenchymal stem cells after myocardial infarction. Chin Med J 125:3472–3478

    CAS  PubMed  Google Scholar 

Download references

Acknowledgement

This study was funded and approved by the Ethics Committee of Hamadan University of Medical Sciences (No. IR.UMSHA.REC.1394.67). The Funding Number is 9403262453.

Supplementary information

Supplementary Table 1—Sequences of primers for qRT-PCR.

Author information

Authors and Affiliations

  1. Endometrium and Endometriosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran

    Asrin Rahimi, Iraj Amiri, Amaneh Mohammadi Roushandeh, Zoleikha Golipour Choshali, Zohreh Alizadeh, Tayebeh Artimani & Sara Soleimani Asl

  2. Anatomy Department, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

    Asrin Rahimi, Iraj Amiri, Amaneh Mohammadi Roushandeh, Zoleikha Golipour Choshali, Zohreh Alizadeh, Tayebeh Artimani & Sara Soleimani Asl

  3. Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

    Saeid Afshar

Authors
  1. Asrin Rahimi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Iraj Amiri
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Amaneh Mohammadi Roushandeh
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zoleikha Golipour Choshali
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Zohreh Alizadeh
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Tayebeh Artimani
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Saeid Afshar
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Sara Soleimani Asl
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sara Soleimani Asl.

Ethics declarations

Conflict of interest

The authors declare no conflict of interest.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 11 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Rahimi, A., Amiri, I., Roushandeh, A.M. et al. Sublethal concentration of H2O2 enhances the protective effect of mesenchymal stem cells in rat model of spinal cord injury. Biotechnol Lett 40, 609–615 (2018). https://doi.org/10.1007/s10529-017-2499-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-017-2499-7

Keywords

Search

Navigation