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Fiber-type distribution and expression of myosin heavy chain isoforms in newborn heterozygous myostatin-knockout pigs

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Abstract

Objectives

To explore the effects of heterozygous myostatin-knockout (MSNT+/−) on muscle characteristics, specifically fiber-type distribution and expression of myosin heavy chain isoforms in pigs.

Results

The fiber cross-sectional area of the semitendinosus and semimembranosus muscles were much larger in MSTN+/− pigs at birth than in wild-type (WT) pigs. MSTN+/− pigs had a higher proportion of fast-type fibers and lower succinate dehydrogenase activity in muscles than WT pigs. The myosin heavy chain IIB mRNA level in both two muscles was ~ threefold higher in MSTN+/− pigs compared with WT pigs.

Conclusion

MSTN+/− pigs exhibit a disproportionate increase in muscle mass and can have a higher body weight due to fiber hypertrophy, a change in the fiber-type distribution, and alteration of myosin heavy chain isoforms levels, leading to more fast glycolytic fibers.

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Acknowledgements

This work was supported by the Science and Technology Development Projects of Jilin Province of China (Grant Number: 20160520057JH) and ToolGen, Inc. We thank the Hanji and Longxing pig farms for providing surrogate pig recipients and their enthusiasm and support for this research.

Supplementary information

Supplementary Table 1—List of primers used for quantitative RT-PCR.

Author information

Author notes

  1. **ao-Xu **ng and Mei-Fu Xuan have been contributed equally to this study.

Authors and Affiliations

  1. Department of Animal Science, Agricultural College, Yanbian University, No. 977 Gongyuan Str., Yanji City, 133002, Jilin Pro, China

    **ao-Xu **ng, Mei-Fu Xuan, Long **, Qing Guo, Zhao-Bo Luo, Jun-**a Wang, Qi-Rong Luo, Guang-Lei Zhang, **-Dan Kang & **-Jun Yin

  2. Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, 133002, Jilin, China

    **ao-Xu **ng, Mei-Fu Xuan, Long **, Qing Guo, Zhao-Bo Luo, Jun-**a Wang, Qi-Rong Luo, Guang-Lei Zhang, Cheng-Du Cui, Zheng-Yun Cui, **-Dan Kang & **-Jun Yin

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  1. **ao-Xu **ng
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  2. Mei-Fu Xuan
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Corresponding author

Correspondence to **-Jun Yin.

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**ng, XX., Xuan, MF., **, L. et al. Fiber-type distribution and expression of myosin heavy chain isoforms in newborn heterozygous myostatin-knockout pigs. Biotechnol Lett 39, 1811–1819 (2017). https://doi.org/10.1007/s10529-017-2422-2

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  • DOI: https://doi.org/10.1007/s10529-017-2422-2

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