Abstract
Phytase is a phosphatase enzyme involved in catalyzing the hydrolysis of phytic acid, which is abundant in plant-derived ingredients that are known to be best alternatives for expensive and scarce fish meal in aquafeeds. In this study, the effect of dietary supplementation of microbial phytase at 250, 500, and 750 FTU/Kg on growth, survival and body composition of freshwater prawn (Macrobrachium rosenbergii) was investigated. The growth and protein efficiency ratio in phytase-incorporated diet-fed prawns were significantly increased when compared to the phytase-free diet-fed prawns. The diet supplemented with 250 FTU microbial phytase-fed prawns showed best growth of 50.84 g in terms of mean weight, compared with the phytase-free diet-fed prawns showing 27.88 g in 90 days trial. In addition, a highest protein efficiency ratio of 2.12 was found in 250 FTU phytase-incorporated-diet-fed prawns, compared with 1.53 in phytase-free diet-fed prawns. Furthermore, there was no significant difference in the survival of prawns fed with phytase-supplemented and phytase-free diets. These results indicate that the dietary supplementation of microbial phytase in prawn diet would be beneficial in better utilization of plant-derived feed ingredients, which are of most promising alternatives to expensive and scarce fish meal in aqua feed formulation.
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References
Abo-state HA, Tahoun AM, Hammouda YA (2009) Effect of replacement of soyabean meal by DDGS combined with commercial phytase on Nile tilapia (Oreochromis niloticus) fingerlings growth performance and feed utilization. Am Eurasian J Agric Environ Sci 5(4):473–479
AOAC (1995) Official methods of analysis, 16th edn. Association of Official Analytical Chemists, Arlington
Baruah K, Pal AK, Sahu NP, Debnath D (2007) Microbial phytase supplementation in rohu, Labeo rohita, diets enhances growth performance and nutrient digestibility. J World Aquacult Soc 38:129–137
Biswas P, Pal AK, Sahu NP, Reddy AK, Prusty AK, Misra S (2007) Lysine and/or phytase supplementation in the diet of Penaeus monodon (Fabricius) juveniles: effect on growth, body composition and lipid profile. Aquaculture 265:253–260
Cheng ZJ, Hardy RW (2002) Effect of microbial phytase on apparent nutrient digestibility of barley, canola meal, wheat and wheat middlings, measured in vivo using rainbow trout (Oncorhynchus mykiss). Aquac Nutr 8:271–277
De Silva SS, Andersons TA (1995) Fish nutrition in aquaculture. Chapman and Hall aquaculture series 1. Chapman and Hall, London
Debnath D, Pal AK, Sahu NP, Jain KK, Yengkokpam S, Mukherjee SC (2005) Effect of dietary microbial phytase supplementation on growth and nutrient digestibility of Pangasius pangasius (Hamilton) fingerlings. Aquac Res 36:180–187
Felt S (1990) Commercial catfish feeding practices. In: Smitherman RO, Tarl D (eds) Proceedings symposium on fisheries and aquaculture. Auburn University, Alabama, September, 1984, pp 131–135
Forster I, Higgs DA, Dosanjh BS, Rowshandeli M, Parr J (1999) Potential for dietary phytase to improve the nutritive value of canola protein concentrate and decrease phosphorus output in rainbow trout (Oncorhynchus mykiss) held in 11 C fresh water. Aquaculture 179:109–125
Francis G, Makkar HPS, Becker K (2001) Antinutritional factors present in plant-derived alternate fish feed ingredients and their effects in fish. Aquaculture 199:197–227
Hastings WH (1976) Fish nutrition and fish feed manufacture. In: FAO technical conference on aquaculture, p. 13, Kyoto, Japan, FIR: Aq. Cof. 76. R 23
Hughes KP, Soares JH Jr (1998) Efficacy of phytase on phosphorus utilization in practical diets fed to striped bass, Morone saxitills. Aquac Nutr 4:133–140
Jackson LS, Li MH, Robinson EH (1996) Use of microbial phytase in channel catfish Ictalurus punctatus diets to improve utilization of phytate phosphorus. J World Aquac Soc 27:309–313
Kornegay ET (1995) Important considerations for using microbial phytase in broiler and turkey diets. In: van Hartingsveldt W, Hessing M, van der Lugt JP, Somers WAC (eds) Proceedings of second symposium on feed enzymes (ESFE2), Noordwijkerhout, Netherlands, TNO Nutrition and Food Research Institute, Zeist, pp 189–197
Laining A, Ishikawa M, Kyaw K, Gao J, Binh NT, Koshio S, Yamaguchi S, Yokoyama S, Koyama J (2011) Dietary calcium/phosphorus ratio influences the efficacy of microbial phytase on growth, mineral digestibility and vertebral mineralization in juvenile tiger puffer, Takifugu rubripes. Aquac Nutr 17:267–277
Li MH, Robinson EH (1997) Microbial phytase can replace inorganic phosphorus supplements in channel catfish Ictalurus punctatus diets. J World Aquac Soc 28:402–406
Murthy HS (1998) Freshwater prawn culture in India. INFOFISH Int 5:30–36
Murthy HS (2005) Recent advances and constraints in hatchery production and grow-out farming of freshwater prawn in India. Fish Chim 25(1):54–56
Nwanna LC, Kolahsa M, Eisenreich R, Schwarz FJ (2008) Pre-treatment of dietary plant feedstuffs with phytase and its effect on growth and mineral concentration in common carp (Cyprinus carpio L.). J Anim Physiol Anim Nutr 92:677–682
Parmer PV, Murthy HS, Tejpal CS, Naveenkumar BT (2012) Effect of brewer’s yeast on immune response of giant freshwater prawn, Macrobrachium rosenbergii, and its resistance to white muscle disease. Aquac Int 20:951–964
Robinson EH, Li MH, Manning BB (2002) Comparison of microbial phytase and dicalcium phosphate for growth and bone mineralization of pond raised channel catfish, Ictalurus punctatus. J Appl Aquac 12:81–88
Rodehutscord M, Pfeffer E (1995) Effects of supplemental microbial phytase on phosphorus digestibility and utilization in rainbow trout (Oncorhynchus mykiss). Water Sci Technol 31:143–147
Sarker PK, Hosokawa H (2009) Effect of phytase on growth and phosphorus utilization in Japanese flounder (Paralichthys olivaceus). Int J Recirc Aquac 10:25–41
Schafer A, Koppe WM, Meyer-Burgdoff KH, Gunther KD (1995) Effects of microbial phytase on the utilization on the utilization of native phosphorus by carp in a diet based on soybean meal. Water Sci Technol 31:149–155
Usmani N, Jafri AK (2002) Influence of dietary acid on the growth, conversion efficiency, and carcass composition of mrigal, Cirrhinus mrigala (Hamilton) fry. J World Aquac Soc 33:199–204
Vandenberg GW, Scott SL, Sarker PK, Dallaire V, de la Noüe J (2011) Encapsulation of microbial phytase: effects on phosphorus bioavailability in rainbow trout (Oncorhynchus mykiss). Anim Feed Sci Technol 169:230–243
Van Weerd JH, Khalaf KHA, Aartsen FJ, Tijssen PAT (1999) Balance trials with African catfish Clarias gariepinus fed phytase-treated soybean meal-based diets. Aqua Nutr 5:135–142
Yan W, Reigh RC, Xu Z (2002) Effects of fungal phytase on utilization of dietary protein and minerals, and dephosphorylation of phytic acid in the alimentary tract of channel catfish Ictalurus punctarus fed an all plant-protein diet. J World Aqua Soc 33:10–22
Yoo GY, Wang XJ, Choi SM, Han KM, Kang JC, Bai SC (2005) Dietary microbial phytase increased the phosphorous digestibility in juvenile Korean rockfish Sebastes schlegeli fed diets containing soybean meal. Aquaculture 243:315–322
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Biradar, S., Shivananda Murthy, H., Patil, P. et al. Dietary supplementation of microbial phytase improves growth and protein efficiency ratio of freshwater prawn (Macrobrachium rosenbergii). Aquacult Int 25, 567–575 (2017). https://doi.org/10.1007/s10499-016-0057-2
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DOI: https://doi.org/10.1007/s10499-016-0057-2