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Yeast microbiota associated with spontaneous sourdough fermentations in the production of traditional wheat sourdough breads of the Abruzzo region (Italy)

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Abstract

The aims of this study were to describe the yeast community of 20 sourdoughs collected from central Italy and to characterize the sourdoughs based on chemical properties. A polyphasic approach consisting of traditional culture-based tests (spore-forming and physiological tests) and molecular techniques (PCR-RFLP, RAPD-PCR, PCR-DGGE) and chemical analysis (total acidity, acids, and sugar contents), was utilized to describe the yeast population and to investigate the chemical composition of the doughs. PCR-RFLP analysis identified 85% of the isolates as Saccharomyces cerevisiae, with the other dominant species being Candida milleri (11%), Candida krusei (2.5%), and Torulaspora delbrueckii (1%). RAPD-PCR analysis, performed with primers M13 and LA1, highlighted intraspecific polymorphism among the S. cerevisiae strains. The diversity of the sourdoughs from the Abruzzo region is reflected in the chemical composition, yeast species, and strain polymorphism. Our approach using a combination of phenotypic and genotypic methods identified the yeast species in the 20 sourdough samples and provided a complete overview of the yeast populations found in sourdoughs from the Abruzzo region.

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References

  • Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402

    Article  CAS  PubMed  Google Scholar 

  • Barnett JA, Payne RW, Yarrow D (2000) Yeasts: characteristics and identification. Cambridge University Press, Cambridge

    Google Scholar 

  • Corsetti A, Gobbetti M, Smacchi E (1996) Antibacterial activity of sourdough lactic acid bacteria: isolation of a bacteriocin-like inhibitory substance from Lactobacillus sanfrancisco C57. Food Microbiol 13:447–456

    Article  CAS  Google Scholar 

  • Corsetti A, Gobbetti M, Rossi J, Haznedari S, Buffa T, Rocchetti G (1998) Batteri lattici e lieviti da frumento tenero e farine biologiche-isolamento ed identificazione. Tecnica Molitoria 49:1–8

    Google Scholar 

  • Corsetti A, Lavermicocca P, Morea M, Baruzzi F, Tosti N, Gobbetti M (2001) Phenotypic and molecular identification and clustering of lactic acid bacteria and yeasts from wheat (species Triticum durum and Triticum aestivum) sourdoughs of Southern Italy. Int J Food Microbiol 64:95–104

    Article  CAS  PubMed  Google Scholar 

  • Corsetti A, Settanni L, Valmorri S, Mastrangelo M, Suzzi G (2007) Identification of subdominant sourdough lactic acid bacteria and their evolution during laboratory-scale fermentations. Food Microbiol 24:592–600

    Article  CAS  PubMed  Google Scholar 

  • de Barros Lopes M, Soden A, Henschke PA, Langridge P (1996) PCR differentiation of commercial yeast strains using intron splice site primers. Appl Environ Microbiol 62:4514–4520

    PubMed  Google Scholar 

  • de Llanos Frutos R, Fernández-Espinar MT, Querol A (2004) Identification of species of the genus Candida by analysis of the 5.8S rRNA gene and the two ribosomal internal transcribed spacers. Antonie Leeuwenhoek 85:175–185

    Article  PubMed  Google Scholar 

  • Ercolini D (2004) PCR-DGGE fingerprinting: novel strategies for detection of microbes in food. J Microbiol Methods 56:297–314

    Article  CAS  PubMed  Google Scholar 

  • Esteve-Zarzoso B, Belloch C, Uruburu F, Querol A (1999) Identification of yeasts by RFLP analysis of the 5.8S rRNA gene and the two ribosomal internal transcribed spacers. Int J Syst Bacteriol 49:329–337

    Google Scholar 

  • Ferchichi M, Valcheva R, Prevost H, Onno B, Dousset X (2007) Molecular identification of the microbiota of French sourdough using temporal temperature gradient gel electrophoresis. Food Microbiol 24:678–686

    Article  CAS  PubMed  Google Scholar 

  • Foschino R, Gallina S, Andrighetto C, Rossetti L, Galli A (2004) Comparison of cultural methods for the identification and molecular investigation of yeasts from sourdoughs for Italian sweet baked products. FEMS Yeast Res 4:609–618

    Article  CAS  PubMed  Google Scholar 

  • Gänzle MG, Ehrmann M, Hammes WP (1998) Modelling of growth of Lactobacillus sanfranciscensis and Candida milleri in response to process parameters of sourdough fermentation. Appl Environ Microbiol 18:277–285

    Google Scholar 

  • Garofalo C, Silvresti G, Aquilanti L, Clementi F (2008) PCR-DGGE analysis of lactic acid bacteria and yeast dynamics during the production processes of three varieties of Panettone. J Appl Microbiol 105:243–254

    Article  CAS  PubMed  Google Scholar 

  • Gatto V, Torriani S (2004) Microbial population changes during sourdough fermentation monitored by DGGE analysis of 16S and 26S rRNA gene fragments. Ann Microbiol 54:31–42

    CAS  Google Scholar 

  • Gobbetti M (1998) The sourdough microflora: interactions of lactic acid bacteria and yeasts. Trends Food Sci Technol 9:267–274

    Article  CAS  Google Scholar 

  • Gobbetti M, Corsetti A, Rossi J, La Rosa F, De Vincenzi S (1994) Identification and clustering of lactic acid bacteria and yeasts from wheat sourdoughs of central Italy. Italian J Food Sci 6:85–94

    CAS  Google Scholar 

  • Gullo M, Romano AD, Pulvirenti A, Giudici P (2003) Candida humilis-dominant species in sourdoughs for the production of durum wheat bran flour bread. Int J Food Microbiol 80:55–59

    Article  CAS  PubMed  Google Scholar 

  • Halm A, Sørensen LA, Jakobsen M (1993) Microbiological and aromatic characteristics of fermented maize dough for ‘Kenkey’ production in Ghana. Int J Food Microbiol 19:135–143

    Article  CAS  PubMed  Google Scholar 

  • Hämmes WP, Gänzle MG (1998) Sourdough breads and related products. In: Wood BJB (ed) Microbiology of fermented foods, vol 1. Blackie Academic and Professional, London, pp 199–216

    Google Scholar 

  • Hammes WP, Vogel RF (1995) The genus Lactobacillus. In: Wood BJB, Holzapfel WH (eds) The genera of lactic acid bacteria. Blackie Academic and Professional, London, pp 19–54

    Google Scholar 

  • Hansen Ǻ, Hansen B (1996) Flavour of sourdough wheat bread crumb. Z Lebens Unters Forsch 202:244–249

    Article  CAS  Google Scholar 

  • Iacumin L, Cecchini F, Manzano M, Osualdini M, Boscolo D, Orlic S, Comi G (2009) Description of the microflora of sourdoughs by culture-dependent and culture-independent methods. Food Microbiol 26:128–135

    Article  CAS  PubMed  Google Scholar 

  • INSOR (Istituto Nazionale di Sociologia Rurale) (2000) Atlante dei prodotti tipici II. Pane. AGRA–RAI–ERI, Rome

  • Kurtzman CP, Fell JW (1998) The yeasts, a Taxonomic Study, 4th edn. Elsevier Science, Amsterdam

    Google Scholar 

  • Kurtzman CP, Robnett CJ (1998) Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequence. Antonie Leeuwenhoek 73:331–371

    Article  CAS  PubMed  Google Scholar 

  • Lodder J, Kreger-Van Rij NJV (1952) The yeasts: a taxonomic study. North Holland, New York, Amsterdam

    Google Scholar 

  • Meroth CB, Hammes WP, Hertel C (2003a) Identification and population dynamics of yeasts in sourdough fermentation process by PCR-Denaturing Gradient Gel Electrophoresis. Appl Environ Microbiol 69:7453–7461

    Article  CAS  PubMed  Google Scholar 

  • Meroth CB, Walter J, Hertel C, Brandt MJ, Hammes WP (2003b) Monitoring the bacterial population dynamics in sourdough fermentation processes by using PCR-Denaturing Gradient Gel Electrophoresis. Appl Environ Microbiol 69:475–482

    Article  CAS  PubMed  Google Scholar 

  • Obiri-Danso K (1994) Microbiological studies on corn dough ferment. Cereal Chem 7:186–188

    Google Scholar 

  • Ottogalli G, Galli A, Foschino R (1996) Italian bakery products obtained with sour dough: characterization of the typical microflora. Adv Food Sci 18:131–144

    Google Scholar 

  • Pulvirent A, Caggia C, Restuccia C, Gullo M, Giudici P (2001) DNA fingerprinting methods used for identification of yeasts isolated from Sicilian sourdough. Ann Microbiol 51:107–120

    Google Scholar 

  • Pulvirenti A, Solieri L, Gullo M, De Vero L, Giudici P (2004) Occurrence and dominance of yeast species in sourdough. Lett Appl Mircobiol 38:113–117

    Article  CAS  Google Scholar 

  • Querol A, Barrio E, Huerta T, Ramon D (1992) Molecular monitoring of wine fermentations conduced by active dry yeast system. Appl Environ Microbiol 58:2948–2953

    CAS  PubMed  Google Scholar 

  • Röcken W (1996) Applied aspects of sourdough fermentation. Adv Food Sci 18:212–216

    Google Scholar 

  • Röcken W, Voysey PA (1995) Sour-dough fermentation in bread making. J Appl Bacteriol Symp 79:38S–48S

    Google Scholar 

  • Rossi J (1996) The yeasts in sourdough. Adv Food Sci 18:201–211

    CAS  Google Scholar 

  • Settanni L, Valmorri S, van Sinderen D, Suzzi G, Paparella A, Corsetti A (2006) Combination of Multiplex PCR and PCR-Denaturing Gradient Gel Electrophoresis for monitoring common sourdough-associated Lactobacillus species. Appl Environ Microbiol 72:3793–3796

    Article  CAS  PubMed  Google Scholar 

  • Stenlid J, Karlsson JO, Hogberg N (1994) Intraspecific genetic variation in Heterobasidium annosum revealed by amplification of minisatellite DNA. Mycol Res 98:57–63

    Article  CAS  Google Scholar 

  • Succi M, Reale A, Andrighetto C, Lombardi A, Sorrentino E, Coppola R (2003) Presence of yeasts in Southern Italian sourdoughs from Triticum aestivum flour. FEMS Microbiol Lett 225:143–148

    Article  CAS  PubMed  Google Scholar 

  • Sugihara TF, Kline L, Miller MW (1971) Microorganisms of the San Francisco sour dough bread process, I, Yeasts responsible for the leavening action. J Appl Microbiol 21:456–458

    CAS  Google Scholar 

  • Valcheva R, Korakl M, Onno B, Prévost H, Ivanova I, Ehrmann MA, Dousset X, Gänzle MG, Vogel RF (2005) Lactobacillus hammesii sp. nov., isolated from French sourdough. Int J Syst Evol Microbiol 55:763–767

    Article  CAS  PubMed  Google Scholar 

  • Valcheva R, Ferchichi M, Korakli M, Ivanova I, Gänzle MG, Vogel RF, Prévos H, Onno B, Dousset X (2006) Lactobacillus nantensis sp. nov., isolated from French wheat sourdough. Int J Syst Evol Microbiol 56:587–591

    Article  CAS  PubMed  Google Scholar 

  • Valmorri S, Settanni L, Suzzi G, Gardini F, Vernocchi P, Corsetti A (2006) Application of a novel polyphasic approach to study the lactobacilli composition of sourdoughs from the Abruzzo region (central Italy). Lett Appl Microbiol 43:343–349

    Article  CAS  PubMed  Google Scholar 

  • Van der Walt JP, Johannsen E (1975) Pichia philgenia sp. nov. Antonie Leeuwenhoek 41:173–177

    Article  PubMed  Google Scholar 

  • Vernocchi P, Valmorri S, Dalai I, Torriani S, Gianotti A, Suzzi G, Guerzoni ME, Mastrocola D, Gardini F (2004a) Characterization of the yeast population involved in the production of a typical Italian bread. J Food Sci 69:182–186

    Article  Google Scholar 

  • Vernocchi P, Valmorri S, Gatto V, Torriani S, Gianotti A, Suzzi G, Guerzoni ME, Gardini F (2004b) A survey on yeast microbiota associated with an Italian traditional sweet-leavened baked good fermentation. Food Res Int 37:469–476

    Article  CAS  Google Scholar 

  • Villa-Carvajal M, Querol A, Belloch C (2006) Identification of species in the genus Pichia by restriction of the internal transcribed spacers (ITS1 and ITS2) and the 5.8S ribosomal DNA gene. Antonie Leeuwehoek 90:171–181

    Article  CAS  Google Scholar 

  • Vogel RF, Knorr R, Müller MRA, Stendel U, Gänzle MG, Ehmann MA (1999) Non-dairy lactic fermentation: the cereal world. Antonie Leeuwenhoek 76:403–411

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Food Science, University of Teramo, Via C.R. Lerici 1, 64023, Mosciano Sant’Angelo, Teramo, Italy

    Sara Valmorri, Rosanna Tofalo, Aldo Corsetti & Giovanna Suzzi

  2. SENFIMIZO Department, Section of Phytopathology and Agricultural Microbiology, University of Palermo, Viale delle Scienze, 4-90128, Palermo, Italy

    Luca Settanni

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  1. Sara Valmorri
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  2. Rosanna Tofalo
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  3. Luca Settanni
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  4. Aldo Corsetti
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  5. Giovanna Suzzi
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Correspondence to Giovanna Suzzi.

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Valmorri, S., Tofalo, R., Settanni, L. et al. Yeast microbiota associated with spontaneous sourdough fermentations in the production of traditional wheat sourdough breads of the Abruzzo region (Italy). Antonie van Leeuwenhoek 97, 119–129 (2010). https://doi.org/10.1007/s10482-009-9392-x

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