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Rodent model of nonarteritic ischemic optic neuropathy and its electrophysiological evaluation

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Abstract

Purpose

Our aim was to establish a rodent model of nonarteritic ischemic optic neuropathy (rNAION).

Methods

To induce rNAION, after administration of Rose Bengal (RB) (2.5 mM), the small vessels of the left optic nerve were photoactivated using a 514-nm argon green laser with about 500-μm spot size for 12 s (RB-laser induction). To evaluate the induction, funduscopic examination, fluorescein angiography (FA), visualization of capillaries within the optic disc, histologic evaluation, and electrophysiological testing were performed.

Results

In the RB-laser-induction eyes, the optic disc became swollen on day 3 followed by atrophy on week 8. FA showed filling defects in the choroid and optic disc at an early stage, followed by hyperfluorescence at a late stage. The capillaries within the optic disc were reduced markedly. Histopathologic examination showed acellular nerve fiber layer (NFL) swelling anterior to the optic disc. The morphologic retinal changes were apparent only in the retinal ganglion cell (RGC) layer, with a reduction in the number of cells. Visually evoked potential (VEP) amplitude decreased significantly, but electroretinography (ERG) showed no significant difference. The positive scotopic threshold response (pSTR) was not reduced on the 1st day but was significantly reduced 3 days after induction.

Conclusions

The findings are similar to human NAION. Therefore, RB-laser induction is well suited to establish the presence of rNAION.

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Acknowledgments

We Shigeki Machida from the University of Iwate for his technical support and invaluable comments on electrophysiology, and Yuichiro Sato from University of Miyazaki for his technical support and valuable comments on histopathology.

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Authors and Affiliations

  1. Department of Ophthalmology, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki, Miyazaki, 889-1692, Japan

    Hideki Chuman, Tomoyuki Maekubo, Takako Osako, Yu Kodama, Michitaka Ishiai & Nobuhisa Nao-i

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  1. Hideki Chuman
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  2. Tomoyuki Maekubo
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  3. Takako Osako
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  4. Yu Kodama
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  5. Michitaka Ishiai
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  6. Nobuhisa Nao-i
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Corresponding author

Correspondence to Hideki Chuman.

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Chuman, H., Maekubo, T., Osako, T. et al. Rodent model of nonarteritic ischemic optic neuropathy and its electrophysiological evaluation. Jpn J Ophthalmol 56, 518–527 (2012). https://doi.org/10.1007/s10384-012-0167-y

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  • DOI: https://doi.org/10.1007/s10384-012-0167-y

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