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Strong synergistic activity and egg hatch inhibition by (E,E)-2,4-decadienal and (E)-2-decenal in Meloidogyne species

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Abstract

Recent research has been focused on the potential of plant natural products for the control of nematodes, but few studies are reported on synergistic interactions and egg hatch inhibition properties of botanicals. Herein, we evaluate the nematicidal activity of (E,E)-2,4-decadienal, 2-undecanone, furfural and (E)-2-decenal alone and in binary mixtures, on different Meloidogyne spp. and growth stages. (E,E)-2,4-decadienal exhibited strong paralysis activity on second-stage M. incognita, M. javanica and M. arenaria juveniles (EC50/4d = 8.06, 9.04 and 12.90 μg mL−1, respectively) and all its binary mixtures demonstrated synergy on all nematode species. (E,E)-2,4-decadienal best inhibited M. incognita undifferentiated egg hatch and J2 release from eggs immersed in 10 μg mL−1 solution. SEM experiments revealed malformations, in the form of constrictions, along the larvae body retained in eggs treated with (E)-2-decenal. Egg masses treated with 1 μg mL−1 solution of (E)-2-decenal released less than 50 % of the eggs if compared with the control. (E)-2-decenal and (E,E)-2,4-decadienal arrested M. incognita life cycle in pot bioassays (EC50 = 77.46 and 114.47 mg kg−1), with the latter additionally promoting tomato growth. Ailanthus altissima powder (AWP) and water extract (AWE), naturally containing (E,E)-2,4-decadienal and (E)-2-decenal, strongly arrested M. incognita life cycle in tomato plants, and AWP was far more active than AWE. Thus, we conclude that (E,E)-2,4-decadienal and (E)-2-decenal are promising nematicidal agents used alone, in artificial blends or as naturally present in botanical soil amendments.

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Acknowledgements

This work was partially financed by Research Committee of University of Thessaly in the frame of Targeted research Action Programs 2012–2013 and also by a scholarship for Ph.D. studies of the University of Cagliari. Special thanks are due to T. Koufakis and AGRIS SA for providing seeds and seedlings and to Tzortzakakis Emmanuel for the nematode species speciments.

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Author notes

  1. Nikoletta Ntalli

    Present address: Laboratory of Biological Control of Pesticides, Department of Pesticides Control & Phytopharmacy, Benaki Phytopathological Institute, 8 Stefanou Delta Street, Kifissia, 14561, Athens, Greece

Authors and Affiliations

  1. Department of Agriculture, Crop Production and Rural Environment, University of Thessaly, Nea Ionia, 38446, Volos, Greece

    Nikoletta Ntalli, Athanasios Thanasenaris, Dorothea Kontea & Nikolaos G. Tsiropoulos

  2. Pesticide Science Laboratory, Faculty of Agriculture Forestry and Natural Environment, School of Agriculture, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece

    Chrisostomos Oplos, Michalis Michailidis & Urania Menkissoglu-Spiroudi

  3. Department of Life and Environmental Sciences, University of Cagliari, via Ospedale 72, 09124, Cagliari, Italy

    Pierluigi Caboni

  4. Electron and Confocal Microscope Laboratory, Faculty of Biology, Adam Mickiewicz University, Poznan, Poland

    Zbigniew Adamski

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  1. Nikoletta Ntalli
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Correspondence to Nikoletta Ntalli.

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Communicated by M. B. Isman.

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Ntalli, N., Oplos, C., Michailidis, M. et al. Strong synergistic activity and egg hatch inhibition by (E,E)-2,4-decadienal and (E)-2-decenal in Meloidogyne species. J Pest Sci 89, 565–579 (2016). https://doi.org/10.1007/s10340-015-0711-x

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