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On-line SPE Using Ionic Liquid-Based Monolithic Column for the Determination of Antihypertensives in Human Plasma

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Abstract

A poly(ionic liquid-glycidylmethacrylate-co-ethyleneglycol dimethacrylate) monolithic column was prepared via in situ free-radical polymerisation and used as an on-line solid-phase extraction column. The obtained monolithic column was characterized by scanning electron microscopy, Fourier transform infrared spectrometer, nitrogen adsorption–desorption measurement, and mercury intrusion porosimetry, which showed a relatively uniform and porous structure. During the on-line solid-phase extraction–high performance liquid chromatography protocol, nifedipine, nitrendipine, and felodipine were separated efficiently from human plasma using a methanol–water linear gradient at a flow rate of 1.0 mL min−1. The extraction efficiency for the targets was satisfactory with no matrix interference. Under the optimized extraction conditions, the linear regression coefficient was >0.9973; precisions for inter- and intra-day assays presented as relative standard deviations were less than 7.29 %, and the accuracy and recovery was in the range of 96–105 and 90–106 %, respectively. As a result, the polymerization monolithic column exhibited high selectivity and good permeability, and it was feasible to be used as an on-line SPE column. Meanwhile, validation assays also demonstrated acceptable sensitivity and precision for simultaneous quantitative screening of nifedipine, nitrendipine, and felodipine in human plasma.

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Acknowledgments

The project was sponsored by the National Natural Science Foundation of China (No. 21505030); the National Science Foundation of Hebei Province (No B2015201024); and the National Science Foundation of Hebei University (Nos. 2013-247, 2014-08).

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Authors and Affiliations

  1. College of Pharmaceutical Sciences, Hebei University, Baoding, 071002, China

    Sha Liu, Shuai He, Ligai Bai & Haiyan Liu

  2. Key Laboratory of Pharmaceutical Quality Control of Hebei Province, Baoding, China

    Sha Liu, Ligai Bai & Haiyan Liu

  3. Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Baoding, 071002, China

    Ligai Bai & Haiyan Liu

  4. Yiling Pharmaceutical Co. Ltd., Shijiazhuang, China

    Cuiling Wang

Authors
  1. Sha Liu
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  2. Cuiling Wang
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  3. Shuai He
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  4. Ligai Bai
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  5. Haiyan Liu
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Correspondence to Ligai Bai or Haiyan Liu.

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Liu, S., Wang, C., He, S. et al. On-line SPE Using Ionic Liquid-Based Monolithic Column for the Determination of Antihypertensives in Human Plasma. Chromatographia 79, 441–449 (2016). https://doi.org/10.1007/s10337-016-3045-9

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  • DOI: https://doi.org/10.1007/s10337-016-3045-9

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