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Production of the Aspergillus aculeatus endo-1,4-β-mannanase in A. niger

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Journal of Industrial Microbiology & Biotechnology

Abstract

The β-mannanase gene (man1) from Aspergillus aculeatus MRC11624 (Izuka) was patented for application in the coffee industry. For production of the enzyme, the gene was originally cloned and expressed in Saccharomyces cerevisiae. However the level of production was found to be economically unfeasible. Here we report a 13-fold increase in enzyme production through the successful expression of β-mannanase of Aspergillus aculeatus MRC11624 in Aspergillus niger under control of the A. niger glyceraldehyde-3-phosphate dehydrogenase promoter (gpd P) and the A. awamori glucoamylase terminator (glaAT). The effect of medium composition on mannanase production was evaluated, and it was found that the glucose concentration and the organic nitrogen source had an effect on both the volumetric enzyme activity and the specific enzyme activity. The highest mannanase activity levels of 16,596 nkat ml−1 and 574 nkat mg−1 dcw were obtained for A. niger D15[man1] when cultivated in a process-viable medium containing corn steep liquor as the organic nitrogen source and high glucose concentrations.

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Acknowledgments

We would like to thank Dr. A. Plüddemann (University of Stellenbosch) for providing plasmid pBS-pyrGamdS and Prof. CAMJJ van den Hondel (TNO, Zeist, The Netherlands) for providing the A. niger D15 strain used as fungal host for this study.

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Authors and Affiliations

  1. CSIR Biosciences, Private Bag X2, Modderfontein, 1645, South Africa

    Petrus J. van Zyl, V. Moodley & R. L. Roth

  2. Department of Microbiology, Stellenbosch University, Private Bag X1, Stellenbosch, 7602, South Africa

    S. H. Rose & W. H. van Zyl

Authors
  1. Petrus J. van Zyl
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  2. V. Moodley
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  3. S. H. Rose
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  4. R. L. Roth
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  5. W. H. van Zyl
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Correspondence to Petrus J. van Zyl.

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van Zyl, P.J., Moodley, V., Rose, S.H. et al. Production of the Aspergillus aculeatus endo-1,4-β-mannanase in A. niger . J Ind Microbiol Biotechnol 36, 611–617 (2009). https://doi.org/10.1007/s10295-009-0551-x

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