Abstract
The Fusarium mycotoxin deoxynivalenol (DON) facilitates fungal spread within wheat tissue and the development of Fusarium head blight disease. The ability of wheat spikelets to resist DON-induced bleaching is genotype-dependent. In wheat cultivar (cv.) CM82036 DON resistance is associated with a quantitative trait locus, Fhb1, located on the short arm of chromosome 3B. Gene expression profiling (microarray and real-time RT-PCR analyses) of DON-treated spikelets of progeny derived from a cross between cv. CM82036 and the DON-susceptible cv. Remus discriminated ten toxin-responsive transcripts associated with the inheritance of DON resistance and Fhb1. These genes do not exclusively map to Fhb1. Based on the putative function of the ten Fhb1-associated transcripts, we discuss how cascades involving classical metabolite biotransformation and sequestration processes, alleviation of oxidative stress and promotion of cell survival might contribute to the host response and defence against DON.
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Acknowledgements
We thank Fany Doustaly for technical assistance. We thank Dr. Hermann Buerstmayr (IFA-Tulln, Austria) and the Wheat Genetics Resource Center at Kansas State University (Manhattan, Kansas, USA) for providing wheat seed. We also thank Dr. Peader O’ Gaora (Conway Institute, University College Dublin, Ireland) for critical reading of the microarray analyses section.
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This research was funded by Science Foundation Ireland (project 03-IN3-B414) and EU FP5 project FUCOMYR (QLRT-2000-02044).
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Walter, S., Brennan, J.M., Arunachalam, C. et al. Components of the gene network associated with genotype-dependent response of wheat to the Fusarium mycotoxin deoxynivalenol. Funct Integr Genomics 8, 421–427 (2008). https://doi.org/10.1007/s10142-008-0089-4
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DOI: https://doi.org/10.1007/s10142-008-0089-4