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Purification and Partial Identification of Novel Antimicrobial Protein from Marine Bacterium Pseudoalteromonas Species Strain X153

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Abstract

A marine bacterium, X153, was isolated from a pebble collected at St. Anne du Portzic (France). By 16S ribosomal DNA gene sequence analysis, X153 strain was identified as a Pseudoalteromonas sp. close to P. piscicida. The crude culture of X153 was highly active against human pathogenic strains involved in dermatologic diseases, and marine bacteria including various ichthyopathogenic Vibrio strains. The active substance occurred both in bacterial cells and in culture supernatant. An antimicrobial protein was purified to homogeneity by a 4-step procedure using size-exclusion and ion-exchange chromatography. The highly purified P-153 protein is anionic, and sodium dodecylsulfate polyacrylamide gel electrophoresis gives an apparent molecular mass of 87 kDa. The X153 bacterium protected bivalve larvae against mortality, following experimental challenges with ichthyopathogenic Vibrio. Pseudoalteromonas sp. X153 may be useful in aquaculture as a probiotic bacterium.

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Acknowledgments

We thank C. Deregnaucourt (USM 504, Museum National d’Histoire Naturelle) for SDS-PAGE. This work was supported in part by the program 97.C.0111 “Recherche de nouveaux agents antibiotiques et probiotiques dans les bacteries marines” of the French ministry for Research and Technology.

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Authors and Affiliations

  1. Laboratoire de Chimie et Biochimie des Substances Naturelles, UMR 5154 CNRS, Museum National d’Histoire Naturelle, USM 502, 63, rue Buffon, Paris, 75005, France

    Arlette Longeon, Jean Peduzzi, Michel Barthelemy & Michele Guyot

  2. Micromer Technopole Brest-Iroise, Brest, 29200, France

    Sophie Corre

  3. Laboratoire de Physiologie des Invertebres, DRV, IFREMER, Plouzane, 29280, France

    Jean-Louis Nicolas

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  1. Arlette Longeon
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  2. Jean Peduzzi
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  4. Sophie Corre
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  5. Jean-Louis Nicolas
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  6. Michele Guyot
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Correspondence to Michele Guyot.

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Longeon, A., Peduzzi, J., Barthelemy, M. et al. Purification and Partial Identification of Novel Antimicrobial Protein from Marine Bacterium Pseudoalteromonas Species Strain X153. Mar Biotechnol 6, 633–641 (2004). https://doi.org/10.1007/s10126-004-3009-1

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  • DOI: https://doi.org/10.1007/s10126-004-3009-1

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