Abstract
Twenty microsatellite markers were first developed from the Japanese sea cucumber Stichopus japonicus using an enrichment protocol. Of the 20 microsatellite loci, 19 loci were polymorphic in the population examined. At these polymorphic loci, the number of alleles per locus varied from 2 to 15, and the observed heterozygosities ranged from 0.03 to 0.97, which is considerably higher than those previously found for allozymes. The high variability of the microsatellite markers identified in this study will make them excellent tools for genetic analyses of S. japonicus.
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References
B. Angers L. Bernatchez (1997) ArticleTitleComplex evolution of a salmonid microsatellite locus and its consequences in inferring allelic divergence from size information Mol Biol Evol 14 IssueID3 230–238 Occurrence Handle9066791
N. Bierne S. Launey Y. Naciri-Graven F. Bonhomme (1998) ArticleTitleEarly effect of inbreeding as revealed by microsatellite analyses on Ostrea edulis larvae Genetics 148 IssueID4 1893–1906 Occurrence Handle9560403
D.F. Callen A.D. Thompson Y. Shen H.A. Phillips R.I. Richards J.C. Mulley G.R. Sutherland (1993) ArticleTitleIncidence and origin of “null” alleles in the (AC)n microsatellite markers Am J Hum Genet 52 922–927 Occurrence Handle8488841
K.L. Carleton J.T. Streelman B.-Y. Lee N. Garnhart M. Kidd T.D. Kocher (2002) ArticleTitleRapid isolation of CA microsatellites from the tilapia genome Anim Genet 33 140–144 Occurrence Handle10.1046/j.1365-2052.2002.00817.x Occurrence Handle12047227
S. Choe Y. Oshima (1961) ArticleTitleOn the morphological and ecological differences between two commercial forms, “green” and “red”, of the Japanese common sea cucumber, Stichopus japonicus SELENKA Nippon Suisan Gakkaishi 27 97–105
A. Gullberg H. Tegelstrom M. Olsson (1997) ArticleTitleMicrosatellite in the sand lizard (Lacerta agilis): description, variation, inheritance, and applicability Biochem Genet 35 281–295 Occurrence Handle10.1023/A:1021801217185 Occurrence Handle9435947
A.G. Jones C.A. Stockwell D. Walker J.C. Avise (1998) ArticleTitleThe molecular basis of the microsatellite null allele from the white sand pupfish J Hered 89 339–342 Occurrence Handle10.1093/jhered/89.4.339
M. Kanno A. Kijima (2002a) ArticleTitleQuantitative and qualitative evaluation on the color variation of the Japanese sea cucumber Stichopus japonicus Suisanzoshoku 50 63–69
M. Kanno A. Kijima (2002b) ArticleTitleHigh genetic variability of isozymes in Japanese sea cucumber Stichopus japonicus Fish Genet Breed Sci 31 7–12
M. Kanno A. Kijima (2003) ArticleTitleGenetic differentiation among three color variants of Japanese sea cucumber Stichopus japonicus Fish Sci 69 IssueID4 806–812 Occurrence Handle10.1046/j.1444-2906.2003.00690.x
S. Launey D. Hedgecock (2001) ArticleTitleHigh genetic load in the Pacific oyster Crassostrea gigas Genetics 159 255–265 Occurrence Handle11560902
Q. Li C. Park A. Kijima (2002) ArticleTitleIsolation and characterization of microsatellite loci in the Pacific abalone, Haliotis discus hannai J Shellfish Res 21 IssueID2 811–815
Q. Li C. Park T. Kobayashi A. Kijima (2003a) ArticleTitleInheritance of microsatellite DNA markers in the Pacific abalone Haliotis discus hannai Mar Biotechnol 5 331–338 Occurrence Handle10.1007/s10126-002-0116-8
Q. Li C. Park A. Kijima (2003b) ArticleTitleAllelic transmission of microsatellites and application to kinship analysis in newly hatched Pacific abalone larvae Fish Sci 69 881–887 Occurrence Handle10.1046/j.1444-2906.2003.00703.x
D.J. McGoldrick D. Hedgecock L.J. English P. Baoprasertkul R.D. Ward (2000) ArticleTitleThe transmission of microsatellite alleles in Australian and North American stocks of the Pacific oyster (Crassostrea gigas) selection and null alleles J Shellfish Res 19 779–788
J. Nelson G. Cooper T Garner P. Schnupf (2002) ArticleTitlePolymorphic markers for the sea cucumber Parastichopus californicus Mol Ecol Notes 2 233–235 Occurrence Handle10.1046/j.1471-8286.2002.00205.x
S. Nishimura (1995) Guide to Seashore Animals of Japan with Color Pictures and Keys, Vol. 2 Hoikusya Osaka, Japan
M. Raymond F. Rousset (1995) ArticleTitlePopulation genetics software for exact tests and ecumenicism J Hered 86 248–249
Y.K. Tam I. Kornfield (1996) ArticleTitleCharacterization of microsatellite markers in Homarus (Crustacea, Decapoda) Mol Mar Biol Biotechnol 5 230–238 Occurrence Handle8817929
L. Zane L. Bargelloni T. Patarnello (2002) ArticleTitleStrategies for microsatellite isolation: a review Mol Ecol 11 1–16 Occurrence Handle10.1046/j.0962-1083.2001.01418.x Occurrence Handle11903900
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Kanno, M., Li, Q. & Kijima, A. Isolation and Characterization of Twenty Microsatellite Loci in Japanese Sea Cucumber (Stichopus japonicus). Mar Biotechnol 7, 179–183 (2005). https://doi.org/10.1007/s10126-004-0006-3
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DOI: https://doi.org/10.1007/s10126-004-0006-3