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Detection of new mutations conferring resistance to linezolid in glycopeptide-intermediate susceptibility Staphylococcus hominis subspecies hominis circulating in an intensive care unit

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Abstract

Glycopeptides and linezolid are the most widely used antibiotics to treat infections by methicillin-resistant Staphylococcus spp. We report the presence of various isolates of methicillin-resistant S. hominis subsp. hominis with resistance to linezolid and reduced susceptibility to glycopeptides. We studied ten blood culture isolates of S. hominis subsp. hominis from nine patients admitted to our hospital. Etest was used to study susceptibility to antibiotics commonly prescribed against staphylococci. Domain V region of the 23S rRNA gene was amplified and sequenced to detect possible mutations that confer resistance to linezolid. Pulsed-field gel electrophoresis (PFGE) was used for the clonality study of isolates. All isolates were resistant to oxacillin, gentamicin, levofloxacin, cotrimoxazole, and linezolid, and susceptible to tigecycline and daptomycin. Nine of the isolates were resistant to erythromycin and clindamycin, and showed heterogeneous resistance to glycopeptides. C2190T, G2603T, and G2474T mutations were detected in domain V of the 23S rRNA gene. PFGE showed the presence of two different clones. This report alerts to the possible appearance of clinical strains of methicillin-resistant staphylococci with intermediate resistance to glycopeptides, resistance to linezolid, and multiple resistance to other second-line antibiotics.

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Authors and Affiliations

  1. Departamento de Microbiologia, Facultad de Medicina, University of Granada, Avda de Madrid 11, 18012, Granada, Spain

    A. Sorlozano & J. Gutierrez

  2. Service of Microbiology, San Cecilio University Hospital, Granada, Spain

    T. Martinez & J. A. Perez-Lopez

  3. Intensive Care Unit, San Cecilio University Hospital, Granada, Spain

    M. E. Yuste

  4. National Microbiology Centre, Carlos III Institute of Health, Madrid, Spain

    A. Vindel & T. Boquete

  5. Preventive Medicine, San Cecilio University Hospital, Granada, Spain

    J. Guillen

Authors
  1. A. Sorlozano
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  2. J. Gutierrez
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  3. T. Martinez
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  4. M. E. Yuste
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  5. J. A. Perez-Lopez
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  6. A. Vindel
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  7. J. Guillen
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Correspondence to J. Gutierrez.

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Sorlozano, A., Gutierrez, J., Martinez, T. et al. Detection of new mutations conferring resistance to linezolid in glycopeptide-intermediate susceptibility Staphylococcus hominis subspecies hominis circulating in an intensive care unit. Eur J Clin Microbiol Infect Dis 29, 73–80 (2010). https://doi.org/10.1007/s10096-009-0823-4

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  • DOI: https://doi.org/10.1007/s10096-009-0823-4

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