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The isolation of Corynebacterium coyleae from clinical samples: clinical and microbiological data

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Abstract

Fourteen Corynebacterium coyleae isolates were recovered from 12 in-patients during a 5-years period. In six patients, the isolates were considered as clinically significant, three definite (sepsis), two probable (sepsis and soft tissue infection), and one possible (post-transfusional bacteremia). In the remaining 6 patients (all neonatal bacteremias), there was not enough data for considering the isolates as clinical significant. API Coryne™ identified all isolates as C. jeikeium, while Biolog GP2™ correctly identified 7 out of the 14 isolates. Definitive identification was achieved in all isolates by the sequencing of a fragment of 724 to 1423 pb of 16S rDNA. Successive isolations from two patients presented identical random amplified polymorphic DNA (RAPD) profiles. All of the isolates were in-vitro-sensitive to β-lactams, gentamicin, rifampin, tetracycline, vancomycin, linezolid, and resistant to clindamycin. Resistance to erythromycin occurred in 83.3% of isolates, all of them presenting phenotype cMLS and harboring the gene ermX.

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Acknowledgments

This study has been supported by the Fondo de Investigaciones Sanitarias, Instituto de Salud Carlos III, Madrid, Spain, research project 03/0534.

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Authors and Affiliations

  1. Department of Clinical Microbiology, Complejo Asistencial de León, Sacyl, Altos de Nava s/n, 24071, León, Spain

    M. I. Fernández-Natal, R. H. Rodríguez-Pollán, J. Blanco & F. Cachón

  2. Bacterial Taxonomy Laboratory, Servicio de Bacteriología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid, Spain

    J. A. Sáez-Nieto & S. Valdezate

  3. Department of Clinical Microbiology, Fundación Jiménez Díaz, Madrid, Spain

    R. Fernández-Roblas

  4. Department of Microbiology, Hospital de Plasencia, Cáceres, Spain

    M. Asencio

  5. Department of Pediatrics, Complejo Asistencial de León, León, Spain

    S. Lapeña

  6. Department of Internal Medicine, Complejo Asistencial de León, León, Spain

    J. M. Guerra

  7. Unit for Research in Medical Microbiology and Antimicrobial Chemotherapy, Fundación Jiménez Díaz, Madrid, Spain

    F. Soriano

Authors
  1. M. I. Fernández-Natal
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  2. J. A. Sáez-Nieto
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  3. R. Fernández-Roblas
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  4. M. Asencio
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  5. S. Valdezate
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  6. S. Lapeña
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  7. R. H. Rodríguez-Pollán
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  8. J. M. Guerra
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  9. J. Blanco
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  10. F. Cachón
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  11. F. Soriano
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Correspondence to M. I. Fernández-Natal.

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Fernández-Natal, M.I., Sáez-Nieto, J.A., Fernández-Roblas, R. et al. The isolation of Corynebacterium coyleae from clinical samples: clinical and microbiological data. Eur J Clin Microbiol Infect Dis 27, 177–184 (2008). https://doi.org/10.1007/s10096-007-0414-1

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  • DOI: https://doi.org/10.1007/s10096-007-0414-1

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