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Altered expression of pyrophosphate: Fructose-6-phosphate 1-phosphotransferase affects the growth of transgenic Arabidopsis plants

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Molecules and Cells

Abstract

Pyrophosphate: fructose-6-phosphate 1-phosphotransferase (PFP) catalyzes the reversible interconversion of fructose-6-phosphate and fructose-1,6-bisphosphate, a key step in the regulation of the metabolic flux toward glycolysis or gluconeogenesis. To examine the role of PFP in plant growth, we have generated transgenic Arabidopsis plants that either overexpress or repress Arabidopsis PFP sub-unit genes. The overexpressing lines displayed increased PFP activity and slightly faster growth relative to wild type plants, although their photosynthetic activities and the levels of metabolites appeared not to have significantly changed. In contrast, the RNAi lines showed significantly retarded growth in parallel with the reduced PFP activity. Analysis of photosynthetic activity revealed that the growth retardation phenotype of the RNAi lines was accompanied by the reduced rates of CO2 assimilation. Microarray analysis of our transgenic plants further revealed that the altered expression of AtPFPβ affects the expression of several genes involved in diverse physiological processes. Our current data thus suggest that PFP is important in carbohydrate metabolism and other cellular processes.

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Authors and Affiliations

  1. Plant Metabolism Research Center and Graduate School of Biotechnology, Kyung Hee University, Yongin, 446-701, Korea

    Hyemin Lim, Man-Ho Cho, Jong-Seong Jeon, Seong Hee Bhoo, Yong-Kook Kwon & Tae-Ryong Hahn

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  1. Hyemin Lim
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  2. Man-Ho Cho
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  3. Jong-Seong Jeon
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  4. Seong Hee Bhoo
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  5. Yong-Kook Kwon
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  6. Tae-Ryong Hahn
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Correspondence to Tae-Ryong Hahn.

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These authors contributed equally to this study.

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Lim, H., Cho, MH., Jeon, JS. et al. Altered expression of pyrophosphate: Fructose-6-phosphate 1-phosphotransferase affects the growth of transgenic Arabidopsis plants. Mol Cells 27, 641–649 (2009). https://doi.org/10.1007/s10059-009-0085-0

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  • DOI: https://doi.org/10.1007/s10059-009-0085-0

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