Abstract
Objectives
Over the past few years, studies about growth factors have been increasingly developed and the knowledge of their role in stimulating cell proliferation and differentiation used for therapeutic purposes. This study aims to compare a platelets concentrate, the plasma rich in growth factors (PRGF) to a control, consisting of cellulose membranes, to evaluate in vitro the cellular adhesion and migration of human osteoblasts (hOb) and understand if the use of platelets concentrates could be an advantage in view of bone tissue regeneration.
Study design
Twenty-seven human donors provided 27 blood samples used to make 54 samples: 27 for PRGF and 27 for the control group. PRGFs and controls were incubated for 48 h in sterility in 1 ml of culture with 105 hOb and hOb in the scaffolds were then quantified.
Results
In PRGF samples, hObs were more numerous than in controls. (T = 6.6964, p < 0.0001).
Conclusions
Human osteoblasts are driven to colonize PRGFs with a greater efficacy than negative controls, probably due to the presence of chemokines and growth factors in PRGFs.
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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. Informed consent was obtained from all individual participants included in the study.
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Brucoli, M., Sonzini, R., Bosetti, M. et al. Plasma rich in growth factors (PRGF) for the promotion of bone cell proliferation and tissue regeneration. Oral Maxillofac Surg 22, 309–313 (2018). https://doi.org/10.1007/s10006-018-0712-z
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DOI: https://doi.org/10.1007/s10006-018-0712-z