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Comparative proteome analysis of high and low cadmium accumulating soybeans under cadmium stress

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Abstract

A comparative proteomic study was performed to unravel the protein networks involved in cadmium stress response in soybean. Ten-day-old seedlings of contrasting cadmium accumulating soybean cultivars—Harosoy (high cadmium accumulator), Fukuyutaka (low cadmium accumulator), and their recombinant inbred line CDH-80 (high cadmium accumulator) were exposed to 100 μM CdCl2 treatment for 3 days. Root growth was found to be affected under cadmium stress in all. Varietal differences at root protein level were evaluated. NADP-dependent alkenal double bond reductase P1 was found to be more abundant in low cadmium accumulating Fukuyutaka. Leaf proteome analysis revealed that differentially expressed proteins were primarily involved in metabolism and energy production. The results indicate that both high and low cadmium accumulating cultivars and CDH-80 share some common defense strategies to cope with the cadmium stress. High abundance of enzymes involved in glycolysis and TCA cycle might help cadmium challenged cells to produce more energy necessary to meet the high energy demand. Moreover, enhanced expressions of photosynthesis related proteins indicate quick utilization of photoassimilates in energy generation. Increased abundance of glutamine synthetase in all might be involved in phytochelatin mediated detoxification of cadmium ions. In addition, increased abundance of antioxidant enzymes, namely superoxide dismutase, ascorbate peroxidase, catalase, ensures cellular protection from reactive oxygen species mediated damages under cadmium stress. Enhanced expression of molecular chaperones in high cadmium accumulating cultivar might be another additional defense mechanism for refolding of misfolded proteins and to stabilize protein structure and function, thus maintain cellular homeostasis.

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Abbreviations

2-DE:

Two-dimensional polyacrylamide gel electrophoresis

CBB:

Coomassie brilliant blue

MS:

Mass spectrometry

pI :

Isoelectric point

IEF:

Isoelectric focusing

ROS:

Reactive oxygen species

LC:

Liquid chromatography

GS:

Glutamine synthetase

GSH:

Glutathione

SOD:

Superoxide dismutase

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Acknowledgments

The author Z.H. thankfully acknowledges the financial support provided through the DST-BOYSCAST fellowship programme, Govt. of India. We thank the National Institute of Crop Science, Tsukuba, Japan for providing all the necessary facilities. The authors also thank Dr. Yohei Nanjo and Dr. Keito Nishazawa for their valuable suggestions.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. National Institute of Crop Science, Kannondai 2-1-18, Tsukuba, 305-8518, Japan

    Zahed Hossain, Makita Hajika & Setsuko Komatsu

  2. Department of Botany, West Bengal State University, Kolkata, 700126, West Bengal, India

    Zahed Hossain

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  1. Zahed Hossain
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  2. Makita Hajika
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  3. Setsuko Komatsu
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Correspondence to Setsuko Komatsu.

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Hossain, Z., Hajika, M. & Komatsu, S. Comparative proteome analysis of high and low cadmium accumulating soybeans under cadmium stress. Amino Acids 43, 2393–2416 (2012). https://doi.org/10.1007/s00726-012-1319-6

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  • DOI: https://doi.org/10.1007/s00726-012-1319-6

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