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Isolation, molecular characterization and evaluation of the pathogenicity of a porcine rotavirus isolated from Jiangsu Province, China

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Abstract

In this study, a porcine rotavirus was isolated from a fecal sample from a diarrheic piglet in Jiangsu Province, China. Rotavirus-specific cytopathic effects were observed after 12 blind passages on MA-104 cells, yielding a virus titer of 106.125 TCID50/ml. By applying an 80 % nucleotide cutoff value and the RotaC2.0 automated genoty** tool, the Vp4 genotype of the new isolate was identified as P[7]. The Vp7 genotype was identified as G[9], lineage VI, and sublineage c. Experimentally infected piglets showed severe diarrhea symptoms 16–24 h post-inoculation, indicating that this new porcine rotavirus isolate is a pathogenic strain.

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Acknowledgements

This work was supported by the Department of Research and Development of Sinovet (Bei**g) Biotechnology Co., Ltd. The research results belonged to Sinovet (Bei**g) Biotechnology Co., Ltd.

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Authors and Affiliations

  1. Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences (CAAS), Bei**g, 130112, People’s Republic of China

    Hewei Zhang & Hua Wu

  2. Sinovet (Bei**g) Biotechnology Co., Ltd, Bei**g, 100085, People’s Republic of China

    Hewei Zhang, Zhuo Zhang, Yufei Wang, **n Wang, Mingqi **a & Hua Wu

  3. College of Pharmaceutical and Biological Engineering, Shenyang University of Chemical Technology, Shenyang, 110142, People’s Republic of China

    Yufei Wang

  4. College of Life Science and Technology, **an University, Guangzhou, 510632, People’s Republic of China

    Zhuo Zhang

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  1. Hewei Zhang
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  3. Yufei Wang
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  4. **n Wang
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Correspondence to Hua Wu.

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Zhang, H., Zhang, Z., Wang, Y. et al. Isolation, molecular characterization and evaluation of the pathogenicity of a porcine rotavirus isolated from Jiangsu Province, China. Arch Virol 160, 1333–1338 (2015). https://doi.org/10.1007/s00705-015-2347-9

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  • DOI: https://doi.org/10.1007/s00705-015-2347-9

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