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Whole-genome sequence analysis of a Korean G11P[25] rotavirus strain identifies several porcine-human reassortant events

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Abstract

A rare rotavirus, RVA/Human-wt/KOR/CAU12-2/2012/G11P[25], was isolated from a 16-year-old female with fever and diarrhea during the 2012 rotavirus surveillance in South Korea using a cell culture system, and its full genome sequence was determined and analyzed. Strain CAU12-2 exhibited a G11-P[25]-I12-R1-C1-M1-A1-N1-T1-E1-H1 genotype constellation. Phylogenetic analysis of this strain revealed that it is a human-porcine reassortant of two distant relatives of the G11 strains circulating in the world. The VP7 and VP4 genes are most closely related to those of human G11P[25] viruses (Dhaka6, KTM368, and N-38 strains) identified in South Asia, whereas the VP1 gene originated from a porcine G11P[7] virus (YM strain) that was identified in South America. The VP6 gene was found to belong to the new genotype I12. This study indicates that the G11-P[25]-I12 genotype was introduced into the South Korean population by interspecies transmissions of human and animal rotaviruses, followed by multiple reassortment events.

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Acknowledgment

This work was supported by a grant from the Next-Generation BioGreen 21 Program (No. PJ009553), Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. Department of Microbiology and Research Center for Medical Sciences, Chung-Ang University College of Medicine, Seoul, South Korea

    Van Thai Than & Wonyong Kim

  2. Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Suwon, South Korea

    Jong-Hwa Park & In Sik Chung

  3. Functional Biomaterial Division, National Academy of Agricultural Science, The Rural Development Administration, Suwon, South Korea

    Jong Bum Kim

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  1. Van Thai Than
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  3. In Sik Chung
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Correspondence to Wonyong Kim.

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Than, V.T., Park, JH., Chung, I.S. et al. Whole-genome sequence analysis of a Korean G11P[25] rotavirus strain identifies several porcine-human reassortant events. Arch Virol 158, 2385–2393 (2013). https://doi.org/10.1007/s00705-013-1720-9

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