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Epstein-Barr virus gene polymorphism in different parts of the same nasopharyngeal carcinoma patient

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Abstract

In most previous studies, comparisons of Epstein-Barr virus (EBV) gene polymorphisms and genotypes were made between strains from tumors and normal throat washings (TWs) from different individuals. However, it remains controversial whether different EBV subtypes are present in different parts of the same NPC patient. In order to address this question, in this study, we compared the genotypes in sets of paired throat washings (TW) and paraffin-embedded tissues of 20 patients with nasopharyngeal carcinoma (NPC), and we found the same genotype in throat washings (TWs) and tumor cells from the same individual in most cases. The subtypes of EBER and EBNA1 genes were furthermore sequenced, and identical EBV strains were identified in tumor tissues and TWs. In conclusion, different sites of the same individual are infected by the same EBV strains, except for a few differences in occasional cases, suggesting that the EBV subtype detected in throat washings is a reasonable guide to the subtype present in the carcinoma tissue.

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Acknowledgements

This work was supported by grants from National Natural Science Foundation of China (NSFC 81171571 and NSFC 30970157) and the Natural Science Foundation of Shandong Province (Y2008C90).

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Authors and Affiliations

  1. Department of Clinical Laboratory, The Second Affiliated Hospital of Qingdao University Medical College, 127 Siliunan Road, Qingdao, 266042, China

    Yahong Nie

  2. Department of Nosocomial Infection, Affiliated Hospital of Qingdao University Medical College, Qingdao, 266003, China

    Yingjuan Sun

  3. Department of Medical Microbiology, Qingdao University Medical College, 38 Dengzhou Road, Qingdao, 266021, China

    Yun Wang & Bing Luo

  4. Department of Clinical Laboratory Diagnostics, Qingdao University Medical College, 38 Dengzhou Road, Qingdao, 266021, China

    Chengyu Liu

  5. Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, USA

    Chengquan Zhao

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  1. Yahong Nie
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Correspondence to Bing Luo.

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Nie, Y., Sun, Y., Wang, Y. et al. Epstein-Barr virus gene polymorphism in different parts of the same nasopharyngeal carcinoma patient. Arch Virol 158, 1031–1037 (2013). https://doi.org/10.1007/s00705-012-1578-2

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  • DOI: https://doi.org/10.1007/s00705-012-1578-2

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