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Specific recognition of protein by deep eutectic solvent–based magnetic β-cyclodextrin molecularly imprinted polymer

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Abstract

A magnetic β-cyclodextrin (MCD) surface molecularly imprinted polymer (MIP) based on deep eutectic solvents (DESs) as cross-linker and functional monomer (MCD@DES-MIP) was successfully synthesized for the specific recognition of bovine hemoglobin (BHb). The adsorption behavior of MCD@DES-MIP for BHb was investigated by adsorption thermodynamics, adsorption kinetics, and pH control experiments. The maximum adsorption capacity of MCD@DES-MIP for BHb under the optimized conditions was 195.94 mg g−1 and the imprinting factor was 4.68. In addition, the competitive adsorption experiments demonstrated that MCD@DES-MIP showed excellent selective extraction ability for BHb in the binary mixture of BHb and bovine serum albumin (BSA). The actual sample analysis manifested that MCD@DES-MIP effectively separated BHb from complex samples. The results of circular dichroism spectra proved that the secondary structure of BHb did not change during elution. The result indicated that MCD@DES-MIP can be used as a new imprinting material for the separation and purification of BHb.

Graphical abstract

Magnetic imprinted microspheres (MCD@DES-MIP) were prepared by free radical polymerization using magnetic β-cyclodextrin (MCD) as carrier, deep eutectic solvents (DESs) as functional monomer and cross-linker. MCD@DES-MIP show high adsorption capacity and excellent selectivity for BHb.

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Funding

This study was financially supported by the National Natural Science Foundation of China (No.21675048).

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Authors and Affiliations

  1. State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, People’s Republic of China

    **yan He, Yuzhi Wang, Heqiong Li, **g Chen, Ziwei Liu & Fangting Xu

  2. Department of Microbiology, College of Basic Medicine, Central South University, Changsha, 410083, People’s Republic of China

    Yigang Zhou

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  1. **yan He
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  2. Yuzhi Wang
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  3. Heqiong Li
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  4. **g Chen
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  5. Ziwei Liu
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  7. Yigang Zhou
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Correspondence to Yuzhi Wang.

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He, X., Wang, Y., Li, H. et al. Specific recognition of protein by deep eutectic solvent–based magnetic β-cyclodextrin molecularly imprinted polymer. Microchim Acta 188, 232 (2021). https://doi.org/10.1007/s00604-021-04887-x

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  • DOI: https://doi.org/10.1007/s00604-021-04887-x

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