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“French fries”-like luminescent metal organic frameworks for the fluorescence determination of cytochrome c released by apoptotic cells and screening of anticancer drug activity

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Abstract

A luminescent metal organic framework was prepared by encapsulating Zn–Ag–In–S quantum dots into “French fries”-like MIL-68(In) metal organic frameworks (ZAISQDs@MIL-68(In)). The ZAISQDs@MIL-68(In) had a maximum excitation wavelength at 370 nm and maximum emission wavelength at 620 nm. It was found that the ZAISQDs@MIL-68(In) was efficiently quenched by cytochrome c (Cyt c), which is an important biomarker of early cell apoptosis. The quenching mechanism was ascribed to be an inner filter effect and dynamic quenching of Cyt c towards the ZAISQDs@MIL-68(In), and the enrichment effect of MIL-68(In). Benefiting from the multiple advantages, ZAISQDs@MIL-68(In) was developed as an assay strategy of Cyt c with logarithmic relation between signal quenching and concentration in the range 0.02 to 3.5 μM. The linear equation was (F0–F)/F0 = 0.5043 + 0.2678 × logcCyt c with a detection limit of 8 nM. Cyt c released by drug induced apoptotic cells was determined by ZAISQDs@MIL-68(In), and this strategy has been utilized for the screening of anticancer drug activity.

Schematic representation of the synthesis of ZAISQDs@MIL-68(In) and its application for Cyt c and screening anticancer drug activity

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Funding

This work was financially supported by the Natural Science Foundation of China (No. 21705050), Pearl River S&T Nova Program of Guangzhou (201806010170).

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Authors and Affiliations

  1. Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, Guangdong, China

    Ruirui **e, Yanyan Liu, Lan Huang, Xun Zou, Jiamin Liu & Peng Zhao

  2. School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, 510641, Guangdong, China

    Peipei Yang, Qingfan Ren & Jia Tao

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  1. Ruirui **e
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Correspondence to Jia Tao or Peng Zhao.

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**e, R., Liu, Y., Yang, P. et al. “French fries”-like luminescent metal organic frameworks for the fluorescence determination of cytochrome c released by apoptotic cells and screening of anticancer drug activity. Microchim Acta 187, 221 (2020). https://doi.org/10.1007/s00604-020-4207-x

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